Xanthoria parietina 46-1-SA22 v1.1

Identified secondary metabolite regions using strictness 'relaxed'

scaffold_1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	NRPS-like	57,786	115,259
Region 1.2	terpene	887,243	916,640
Region 1.3	T1PKS	1,235,395	1,302,282	1,3,6,8-tetrahydroxynaphthalene	Polyketide	100%
Region 1.4	NRPS	2,103,638	2,165,468	pneumocandin B0/pneumocandin A0	Polyketide+NRP	6%
Region 1.5	NRPS,terpene	3,639,740	3,714,932	squalestatin S1	Terpene	40%
Region 1.6	NRPS-like	3,755,055	3,819,503
Region 1.7	terpene	3,857,226	3,888,494

scaffold_10

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	238,070	308,666

scaffold_11

Region	Type	From	To
Region 3.1	terpene	125,897	157,417
Region 3.2	T1PKS	536,906	605,680
Region 3.3	fungal-RiPP-like	1,127,491	1,219,197

scaffold_12

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	T1PKS	52,758	82,696	solanapyrone D	Polyketide	33%
Region 4.2	T1PKS,NRPS	295,352	369,770
Region 4.3	terpene	421,673	453,199

scaffold_14

Region	Type	From	To
Region 6.1	fungal-RiPP-like	127,569	218,302
Region 6.2	NRPS	551,181	614,955
Region 6.3	NRPS-like	703,532	767,221

scaffold_15

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	T1PKS	59,119	142,459	oxyjavanicin	Polyketide	25%
Region 7.2	T1PKS	163,981	197,608
Region 7.3	T1PKS	390,924	453,872
Region 7.4	T1PKS	466,461	534,379

scaffold_17

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	terpene	702,005	733,392

scaffold_18

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	terpene	64,985	97,570

scaffold_19

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	NRPS-like	75,386	138,884	peramine	NRP	100%
Region 11.2	T3PKS	344,227	405,838

scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	fungal-RiPP-like	292,125	378,729
Region 12.2	T1PKS	1,792,033	1,858,995	cichorine	Polyketide	100%
Region 12.3	fungal-RiPP-like	2,049,040	2,139,759
Region 12.4	T1PKS	2,671,445	2,739,680	solanapyrone D	Polyketide	50%

scaffold_20

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	T1PKS,NRPS-like	314,929	495,028

scaffold_23

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	NRPS	1	66,562
Region 16.2	NRPS-like	68,232	128,483
Region 16.3	T1PKS,NRPS-like	155,226	274,898	duclauxin	Polyketide	21%

scaffold_24

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	NRP-metallophore,NRPS	121,424	186,175

scaffold_3

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	terpene	683,887	715,847
Region 23.2	NRPS,T1PKS	1,212,804	1,285,577	phyllostictine A/phyllostictine B	NRP+Polyketide	50%

scaffold_4

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	T1PKS	162,550	214,667
Region 34.2	T1PKS,NRPS	427,083	501,891	AbT1	NRP	100%
Region 34.3	NRPS-like	638,786	699,715
Region 34.4	NRPS-like	1,802,695	1,865,928

scaffold_5

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	T1PKS	778,480	841,769	depudecin	Polyketide:Iterative type I polyketide	50%
Region 35.2	fungal-RiPP-like	895,227	983,652
Region 35.3	T1PKS,terpene	1,041,023	1,107,843
Region 35.4	fungal-RiPP-like	1,564,085	1,654,754

scaffold_6

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	T1PKS	79,544	154,981
Region 36.2	NRPS-like	382,876	446,643
Region 36.3	T1PKS	1,455,895	1,521,444	TAN-1612/1-(2,3,5,10-tetrahydroxy-7-methoxy-4-oxo-1,2,3,4-tetrahydroanthracen-2-yl)pentane-2,4-dione/desmethyl TAN-1612	Polyketide:Iterative type I polyketide	60%

scaffold_7

Region	Type	From	To	Most similar known cluster		Similarity
Region 37.1	T1PKS	1,094,686	1,161,118	epipyrone A	Polyketide	75%
Region 37.2	NRPS,T1PKS	1,581,068	1,675,968	1,3,6,8-tetrahydroxynaphthalene	Polyketide	100%

scaffold_8

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	NRPS-like	108,040	165,891
Region 38.2	T1PKS	500,292	568,283	trichoxide	Polyketide	25%
Region 38.3	NRPS-like	1,182,406	1,247,220

scaffold_9

Region	Type	From	To	Most similar known cluster		Similarity
Region 39.1	NRPS-like	128,642	191,824

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	NRPS-like	57,786	115,259
Region 1.2	terpene	887,243	916,640
Region 1.3	T1PKS	1,235,395	1,302,282	1,3,6,8-tetrahydroxynaphthalene	Polyketide	100%
Region 1.4	NRPS	2,103,638	2,165,468	pneumocandin B0/pneumocandin A0	Polyketide+NRP	6%
Region 1.5	NRPS,terpene	3,639,740	3,714,932	squalestatin S1	Terpene	40%
Region 1.6	NRPS-like	3,755,055	3,819,503
Region 1.7	terpene	3,857,226	3,888,494
Region 2.1	NRPS	238,070	308,666
Region 3.1	terpene	125,897	157,417
Region 3.2	T1PKS	536,906	605,680
Region 3.3	fungal-RiPP-like	1,127,491	1,219,197
Region 4.1	T1PKS	52,758	82,696	solanapyrone D	Polyketide	33%
Region 4.2	T1PKS,NRPS	295,352	369,770
Region 4.3	terpene	421,673	453,199
Region 6.1	fungal-RiPP-like	127,569	218,302
Region 6.2	NRPS	551,181	614,955
Region 6.3	NRPS-like	703,532	767,221
Region 7.1	T1PKS	59,119	142,459	oxyjavanicin	Polyketide	25%
Region 7.2	T1PKS	163,981	197,608
Region 7.3	T1PKS	390,924	453,872
Region 7.4	T1PKS	466,461	534,379
Region 9.1	terpene	702,005	733,392
Region 10.1	terpene	64,985	97,570
Region 11.1	NRPS-like	75,386	138,884	peramine	NRP	100%
Region 11.2	T3PKS	344,227	405,838
Region 12.1	fungal-RiPP-like	292,125	378,729
Region 12.2	T1PKS	1,792,033	1,858,995	cichorine	Polyketide	100%
Region 12.3	fungal-RiPP-like	2,049,040	2,139,759
Region 12.4	T1PKS	2,671,445	2,739,680	solanapyrone D	Polyketide	50%
Region 13.1	T1PKS,NRPS-like	314,929	495,028
Region 16.1	NRPS	1	66,562
Region 16.2	NRPS-like	68,232	128,483
Region 16.3	T1PKS,NRPS-like	155,226	274,898	duclauxin	Polyketide	21%
Region 17.1	NRP-metallophore,NRPS	121,424	186,175
Region 23.1	terpene	683,887	715,847
Region 23.2	NRPS,T1PKS	1,212,804	1,285,577	phyllostictine A/phyllostictine B	NRP+Polyketide	50%
Region 34.1	T1PKS	162,550	214,667
Region 34.2	T1PKS,NRPS	427,083	501,891	AbT1	NRP	100%
Region 34.3	NRPS-like	638,786	699,715
Region 34.4	NRPS-like	1,802,695	1,865,928
Region 35.1	T1PKS	778,480	841,769	depudecin	Polyketide:Iterative type I polyketide	50%
Region 35.2	fungal-RiPP-like	895,227	983,652
Region 35.3	T1PKS,terpene	1,041,023	1,107,843
Region 35.4	fungal-RiPP-like	1,564,085	1,654,754
Region 36.1	T1PKS	79,544	154,981
Region 36.2	NRPS-like	382,876	446,643
Region 36.3	T1PKS	1,455,895	1,521,444	TAN-1612/1-(2,3,5,10-tetrahydroxy-7-methoxy-4-oxo-1,2,3,4-tetrahydroanthracen-2-yl)pentane-2,4-dione/desmethyl TAN-1612	Polyketide:Iterative type I polyketide	60%
Region 37.1	T1PKS	1,094,686	1,161,118	epipyrone A	Polyketide	75%
Region 37.2	NRPS,T1PKS	1,581,068	1,675,968	1,3,6,8-tetrahydroxynaphthalene	Polyketide	100%
Region 38.1	NRPS-like	108,040	165,891
Region 38.2	T1PKS	500,292	568,283	trichoxide	Polyketide	25%
Region 38.3	NRPS-like	1,182,406	1,247,220
Region 39.1	NRPS-like	128,642	191,824

No secondary metabolite regions were found in these records:: scaffold_13; scaffold_16; scaffold_21; scaffold_22; scaffold_25; scaffold_26; scaffold_27; scaffold_28; scaffold_29; scaffold_30; scaffold_31; scaffold_32; scaffold_33; scaffold_34; scaffold_35; scaffold_36; scaffold_37; scaffold_38; scaffold_39

Compact view

scaffold_1 - Region 1 - NRPS-like

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Location: 57,786 - 115,259 nt. (total: 57,474 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 57785 - 115259:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1592286

AMP-binding (37..347): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PAGILCQ--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_1 - Region 2 - terpene

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Location: 887,243 - 916,640 nt. (total: 29,398 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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transport-related genes

regulatory genes

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resistance

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_1 - Region 3 - T1PKS

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Location: 1,235,395 - 1,302,282 nt. (total: 66,888 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1235394 - 1302282:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1655435

PKS_AT (911..1206): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 130.0
inactive: 80.1
Methoxymalonyl-CoA: 58.7
Methylmalonyl-CoA: 56.7
Propionyl-CoA: 29.5
Isobutyryl-CoA: 24.1
2-Methylbutyryl-CoA: 19.8
CHC-CoA: 18.2
trans-1,2-CPDA: 17.2
Ethylmalonyl-CoA: 16.6
fatty_acid: 14.7
Acetyl-CoA: 13.8
Benzoyl-CoA: 12.9
3-Methylbutyryl-CoA: 11.3

scaffold_1 - Region 4 - NRPS

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Location: 2,103,638 - 2,165,468 nt. (total: 61,831 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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transport-related genes

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resistance

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 4, location 2103637 - 2165468:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - X - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

95730

Search NORINE for peptide: strict or relaxed

AMP-binding (245..644): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVSCTGVVWK
Nearest Stachelhaus code(s):: dhLys DVSETGVCEK (38% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1317..1713): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASLISLIWK
Nearest Stachelhaus code(s):: Val DASQIGGIYK (56% 8� match); Tyr DASTIAGIGK (50% 8� match); Ala DILQISLIYK (47% 8� match); Ser DVWHISLIDK (44% 8� match); Cys DLYNLSLIWK (38% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (2399..2793): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALLVGAVVK
Nearest Stachelhaus code(s):: Leu DALLVGAVVK (50% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_1 - Region 5 - NRPS,terpene

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Location: 3,639,740 - 3,714,932 nt. (total: 75,193 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 3639739 - 3714932:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Arg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 3639739 - 3704772:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1656305

Search NORINE for peptide: strict or relaxed

AMP-binding (620..1021): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSDTGAPTK
Nearest Stachelhaus code(s):: Arg DVSDTGAPTK (91% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_1 - Region 6 - NRPS-like

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Location: 3,755,055 - 3,819,503 nt. (total: 64,449 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 8, location 3755054 - 3819503:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1571124

AMP-binding (10..429): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IHNTIGRMAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

1570850

AMP-binding (250..648): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLLIASFTK
Nearest Stachelhaus code(s):: Ser DLWNIASIWK (35% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_1 - Region 7 - terpene

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Location: 3,857,226 - 3,888,494 nt. (total: 31,269 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_10 - Region 1 - NRPS

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Location: 238,070 - 308,666 nt. (total: 70,597 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 238069 - 308666:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1661569

Search NORINE for peptide: strict or relaxed

AMP-binding (1047..1467): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLLLALGFVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (2133..2544): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSWVDGSIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (62% 8� match); Asp DSWKLGVVDK (50% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_11 - Region 1 - terpene

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Location: 125,897 - 157,417 nt. (total: 31,521 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_11 - Region 2 - T1PKS

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Location: 536,906 - 605,680 nt. (total: 68,775 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 536905 - 605680:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1598261

AMP-binding (51..360): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DANFVGFSFK
Nearest Stachelhaus code(s):: Val DAMFVGGVFK (41% 8� match)
Stachelhaus code match:: 70% (weak)

1662117

PKS_AT (606..928): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 81.8
Methoxymalonyl-CoA: 77.6
Malonyl-CoA: 75.4
Isobutyryl-CoA: 60.8
Ethylmalonyl-CoA: 47.8
inactive: 32.8
2-Methylbutyryl-CoA: 26.2
fatty_acid: 20.8
Propionyl-CoA: 19.1
Benzoyl-CoA: 17.1
trans-1,2-CPDA: 16.0
CHC-CoA: 13.8
3-Methylbutyryl-CoA: 12.8
Acetyl-CoA: 0.0

scaffold_11 - Region 3 - fungal-RiPP-like

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Location: 1,127,491 - 1,219,197 nt. (total: 91,707 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_12 - Region 1 - T1PKS

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Location: 52,758 - 82,696 nt. (total: 29,939 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 52757 - 82696:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1598433

PKS_AT (661..978): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 66.2
Methoxymalonyl-CoA: 64.2
Malonyl-CoA: 50.3
Propionyl-CoA: 38.9
Ethylmalonyl-CoA: 35.7
trans-1,2-CPDA: 30.0
Isobutyryl-CoA: 19.8
fatty_acid: 18.4
2-Methylbutyryl-CoA: 18.3
CHC-CoA: 17.9
Benzoyl-CoA: 14.2
inactive: 13.7
3-Methylbutyryl-CoA: 6.6
Acetyl-CoA: 0.0

scaffold_12 - Region 2 - NRPS,T1PKS

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Location: 295,352 - 369,770 nt. (total: 74,419 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 295351 - 369770:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 295351 - 362848:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 307346 - 369770:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1682944

PKS_AT (591..911): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.6
Malonyl-CoA: 73.6
Methoxymalonyl-CoA: 67.1
Ethylmalonyl-CoA: 56.9
Isobutyryl-CoA: 50.6
Propionyl-CoA: 34.6
inactive: 30.5
2-Methylbutyryl-CoA: 22.0
trans-1,2-CPDA: 17.3
Acetyl-CoA: 13.4
Benzoyl-CoA: 10.9
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

330173

AMP-binding (27..301): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SMSAFGGPMK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_12 - Region 3 - terpene

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Location: 421,673 - 453,199 nt. (total: 31,527 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_14 - Region 1 - fungal-RiPP-like

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Location: 127,569 - 218,302 nt. (total: 90,734 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_14 - Region 2 - NRPS

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Location: 551,181 - 614,955 nt. (total: 63,775 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 551180 - 614955:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1663250

Search NORINE for peptide: strict or relaxed

AMP-binding (56..458): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: AMFACSGPIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_14 - Region 3 - NRPS-like

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Location: 703,532 - 767,221 nt. (total: 63,690 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 3, location 703531 - 767221:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

469322

AMP-binding (43..338): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PMLYAVE-MK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_15 - Region 1 - T1PKS

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Location: 59,119 - 142,459 nt. (total: 83,341 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 59118 - 142459:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (mal)
Non-colinear ordering:: 1663406 + 1583226

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 59118 - 113157:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 100436 - 142459:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1663406

PKS_AT (914..1208): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 94.8
Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 48.4
inactive: 46.6
2-Methylbutyryl-CoA: 22.3
Propionyl-CoA: 21.8
fatty_acid: 21.7
Isobutyryl-CoA: 20.1
3-Methylbutyryl-CoA: 16.1
Ethylmalonyl-CoA: 14.5
Benzoyl-CoA: 14.3
CHC-CoA: 13.3
Acetyl-CoA: 11.4
trans-1,2-CPDA: 9.6

1583226

PKS_AT (390..694): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.4
inactive: 49.3
Methylmalonyl-CoA: 45.1
Methoxymalonyl-CoA: 44.1
Benzoyl-CoA: 21.3
fatty_acid: 19.2
Isobutyryl-CoA: 14.5
Ethylmalonyl-CoA: 9.4
Propionyl-CoA: 6.8
Acetyl-CoA: 5.2
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_15 - Region 2 - T1PKS

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Location: 163,981 - 197,608 nt. (total: 33,628 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_15 - Region 3 - T1PKS

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Location: 390,924 - 453,872 nt. (total: 62,949 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 5, location 390923 - 453872:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1646484

PKS_AT (541..831): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 60.6
inactive: 42.5
Methoxymalonyl-CoA: 38.5
Methylmalonyl-CoA: 34.0
fatty_acid: 18.9
Benzoyl-CoA: 15.4
Propionyl-CoA: 13.6
Isobutyryl-CoA: 13.1
CHC-CoA: 11.9
Acetyl-CoA: 9.7
Ethylmalonyl-CoA: 9.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_15 - Region 4 - T1PKS

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Location: 466,461 - 534,379 nt. (total: 67,919 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 6, location 466460 - 534379:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1617780

PKS_AT (520..810): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; Propionyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 48.6
Isobutyryl-CoA: 22.0
Propionyl-CoA: 13.0
inactive: 10.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.2
Malonyl-CoA: -1.4
Benzoyl-CoA: -2.7
Ethylmalonyl-CoA: -2.9

scaffold_17 - Region 1 - terpene

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Location: 702,005 - 733,392 nt. (total: 31,388 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_18 - Region 1 - terpene

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Location: 64,985 - 97,570 nt. (total: 32,586 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_19 - Region 1 - NRPS-like

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Location: 75,386 - 138,884 nt. (total: 63,499 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomer predictions

1618354

AMP-binding (33..430): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GGAFMAFPSK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_19 - Region 2 - T3PKS

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Location: 344,227 - 405,838 nt. (total: 61,612 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_2 - Region 1 - fungal-RiPP-like

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Location: 292,125 - 378,729 nt. (total: 86,605 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_2 - Region 2 - T1PKS

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Location: 1,792,033 - 1,858,995 nt. (total: 66,963 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 1792032 - 1858995:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1572549

PKS_AT (562..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 86.7
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 58.4
Isobutyryl-CoA: 39.8
Propionyl-CoA: 37.1
inactive: 35.3
Ethylmalonyl-CoA: 33.5
2-Methylbutyryl-CoA: 28.1
fatty_acid: 18.3
Benzoyl-CoA: 12.9
3-Methylbutyryl-CoA: 12.0
trans-1,2-CPDA: 9.8
CHC-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_2 - Region 3 - fungal-RiPP-like

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Location: 2,049,040 - 2,139,759 nt. (total: 90,720 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_2 - Region 4 - T1PKS

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Location: 2,671,445 - 2,739,680 nt. (total: 68,236 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 4, location 2671444 - 2739680:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1594281

PKS_AT (638..953): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 56.5
Methoxymalonyl-CoA: 50.1
Malonyl-CoA: 40.2
Isobutyryl-CoA: 33.3
Ethylmalonyl-CoA: 23.5
Propionyl-CoA: 22.8
fatty_acid: 14.4
CHC-CoA: 9.8
Benzoyl-CoA: 9.8
trans-1,2-CPDA: 9.1
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_20 - Region 1 - NRPS-like,T1PKS

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Location: 314,929 - 495,028 nt. (total: 180,100 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 314928 - 495028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (X) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 314928 - 377254:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 371184 - 434691:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 427377 - 495028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1674999

PKS_AT (526..840): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Isobutyryl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 102.1
Isobutyryl-CoA: 91.0
Methoxymalonyl-CoA: 90.9
Malonyl-CoA: 71.7
Ethylmalonyl-CoA: 55.3
Benzoyl-CoA: 50.4
Propionyl-CoA: 42.4
trans-1,2-CPDA: 40.0
3-Methylbutyryl-CoA: 36.4
inactive: 34.9
Acetyl-CoA: 26.1
2-Methylbutyryl-CoA: 24.1
fatty_acid: 17.8
CHC-CoA: 17.3

884094

AMP-binding (32..466): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMCKAACMK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

1665018

PKS_AT (522..837): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 96.2
Methoxymalonyl-CoA: 85.8
Ethylmalonyl-CoA: 64.4
Isobutyryl-CoA: 63.3
Malonyl-CoA: 62.2
Propionyl-CoA: 44.1
2-Methylbutyryl-CoA: 36.7
trans-1,2-CPDA: 32.6
Benzoyl-CoA: 29.0
fatty_acid: 22.6
CHC-CoA: 18.3
3-Methylbutyryl-CoA: 16.5
inactive: 13.8
Acetyl-CoA: 1.6

scaffold_23 - Region 1 - NRPS

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Location: 1 - 66,562 nt. (total: 66,562 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 0 - 66562:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

926696

AMP-binding (25..304): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IRRIMGAITK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

1675310

AMP-binding (472..855): X

nrpys: Phe, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARGTVSQLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1496..1885): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQIIGVMVK
Nearest Stachelhaus code(s):: Leu DAWIIGAIVK (50% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_23 - Region 2 - NRPS-like

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Location: 68,232 - 128,483 nt. (total: 60,252 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomer predictions

1586296

AMP-binding (293..688): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVMLVGCACK
Nearest Stachelhaus code(s):: Leu DAMLVGAACK (56% 8� match)
Stachelhaus code match:: 80% (moderate)

scaffold_23 - Region 3 - NRPS-like,T1PKS

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Location: 155,226 - 274,898 nt. (total: 119,673 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 3, location 155225 - 274898:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 155225 - 221923:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 210739 - 274898:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

935663

PKS_AT (917..1215): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 81.3
Methoxymalonyl-CoA: 51.7
inactive: 47.3
Methylmalonyl-CoA: 46.9
Propionyl-CoA: 23.9
Isobutyryl-CoA: 20.0
fatty_acid: 16.3
Benzoyl-CoA: 14.9
2-Methylbutyryl-CoA: 13.8
CHC-CoA: 13.0
Ethylmalonyl-CoA: 11.9
Acetyl-CoA: 10.0
3-Methylbutyryl-CoA: 9.2
trans-1,2-CPDA: 0.0

937737

AMP-binding (247..660): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVITCGIICK
Nearest Stachelhaus code(s):: Phe DVSTCGCIIK (41% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_24 - Region 1 - NRP-metallophore,NRPS

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Location: 121,424 - 186,175 nt. (total: 64,752 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 121423 - 186175:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1586413

Search NORINE for peptide: strict or relaxed

AMP-binding (253..652): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQHTITVVK
Nearest Stachelhaus code(s):: Cys DVQHTITVVK (82% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1374..1757): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVASGGAIGK
Nearest Stachelhaus code(s):: Val DPLSTGAIGK (74% 8� match); E-ahmohOrn DVGGGGVIGK (71% 8� match); Arg DVENVGAIGK (56% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_3 - Region 1 - terpene

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Location: 683,887 - 715,847 nt. (total: 31,961 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_3 - Region 2 - NRPS,T1PKS

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Location: 1,212,804 - 1,285,577 nt. (total: 72,774 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 1212803 - 1285577:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1023725

PKS_AT (553..875): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 82.8
Methoxymalonyl-CoA: 69.9
Ethylmalonyl-CoA: 60.5
Malonyl-CoA: 34.6
Isobutyryl-CoA: 34.0
Propionyl-CoA: 31.0
trans-1,2-CPDA: 27.3
fatty_acid: 26.5
Benzoyl-CoA: 25.9
2-Methylbutyryl-CoA: 20.0
CHC-CoA: 16.2
3-Methylbutyryl-CoA: 9.7
inactive: 0.0
Acetyl-CoA: 0.0

AMP-binding (3181..3593): X

nrpys: MalAc

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: NHDKFGSISK
Nearest Stachelhaus code(s):: MalAc NHDKFGSISK (100% 8� match); Phe NHDKFGSISK (97% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_4 - Region 1 - T1PKS

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Location: 162,550 - 214,667 nt. (total: 52,118 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 162549 - 214667:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1698587

PKS_AT (579..898): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 88.4
Methoxymalonyl-CoA: 85.5
Methylmalonyl-CoA: 85.0
Ethylmalonyl-CoA: 54.3
Isobutyryl-CoA: 51.3
inactive: 31.5
Propionyl-CoA: 30.9
2-Methylbutyryl-CoA: 29.7
3-Methylbutyryl-CoA: 20.1
trans-1,2-CPDA: 17.9
fatty_acid: 15.7
CHC-CoA: 12.9
Benzoyl-CoA: 12.1
Acetyl-CoA: 0.0

scaffold_4 - Region 2 - NRPS,T1PKS

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Location: 427,083 - 501,891 nt. (total: 74,809 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 427082 - 501891:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1658288

Search NORINE for peptide: strict or relaxed

PKS_AT (562..899): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 69.7
Methoxymalonyl-CoA: 65.7
Malonyl-CoA: 53.4
Ethylmalonyl-CoA: 46.8
Isobutyryl-CoA: 42.8
Benzoyl-CoA: 34.5
Propionyl-CoA: 25.1
inactive: 14.8
fatty_acid: 11.6
trans-1,2-CPDA: 10.9
3-Methylbutyryl-CoA: 10.9
2-Methylbutyryl-CoA: 9.4
CHC-CoA: 6.4
Acetyl-CoA: 4.5

AMP-binding (3513..3910): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYLCGSVAK
Nearest Stachelhaus code(s):: Cyc-D-Gly DAYLWGGVFK (53% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_4 - Region 3 - NRPS-like

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Location: 638,786 - 699,715 nt. (total: 60,930 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 3, location 638785 - 699715:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1089012

AMP-binding (23..424): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EGCVAGIMMK
Nearest Stachelhaus code(s):: Ile DPCVAGVMLK (35% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_4 - Region 4 - NRPS-like

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Location: 1,802,695 - 1,865,928 nt. (total: 63,234 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 4, location 1802694 - 1865928:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1605498

AMP-binding (24..349): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SAGCCGYLLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_5 - Region 1 - T1PKS

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Location: 778,480 - 841,769 nt. (total: 63,290 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 778479 - 841769:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1702252

PKS_AT (561..856): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 66.0
Methoxymalonyl-CoA: 53.2
Malonyl-CoA: 53.2
Isobutyryl-CoA: 42.2
Ethylmalonyl-CoA: 37.4
Propionyl-CoA: 23.8
3-Methylbutyryl-CoA: 20.2
inactive: 17.3
Benzoyl-CoA: 16.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_5 - Region 2 - fungal-RiPP-like

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Location: 895,227 - 983,652 nt. (total: 88,426 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_5 - Region 3 - T1PKS,terpene

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Location: 1,041,023 - 1,107,843 nt. (total: 66,821 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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Predicted core structure(s)

For candidate cluster 3, location 1041022 - 1107843:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1615524

PKS_AT (884..1177): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 92.3
inactive: 46.5
Methylmalonyl-CoA: 40.7
Methoxymalonyl-CoA: 39.5
Benzoyl-CoA: 21.6
2-Methylbutyryl-CoA: 16.2
3-Methylbutyryl-CoA: 16.0
Isobutyryl-CoA: 15.4
CHC-CoA: 14.4
Propionyl-CoA: 14.2
Ethylmalonyl-CoA: 13.0
Acetyl-CoA: 12.6
trans-1,2-CPDA: 8.5
fatty_acid: 0.0

scaffold_5 - Region 4 - fungal-RiPP-like

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Location: 1,564,085 - 1,654,754 nt. (total: 90,670 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_6 - Region 1 - T1PKS

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Location: 79,544 - 154,981 nt. (total: 75,438 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 79543 - 154981:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (pk)
Non-colinear ordering:: 1669864 + 1249723

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1669864

PKS_AT (597..916): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 74.6
Methoxymalonyl-CoA: 72.9
Malonyl-CoA: 67.1
Isobutyryl-CoA: 26.0
Propionyl-CoA: 23.2
Benzoyl-CoA: 15.3
CHC-CoA: 14.4
trans-1,2-CPDA: 9.5
inactive: 8.8
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.7

1249723

PKS_AT (550..845): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%; 2-Methylbutyryl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 33.2
Methoxymalonyl-CoA: 30.8
Propionyl-CoA: 22.1
Malonyl-CoA: 18.9
CHC-CoA: 13.6
Benzoyl-CoA: 12.2
Isobutyryl-CoA: 11.8
Ethylmalonyl-CoA: 11.2
2-Methylbutyryl-CoA: 9.2
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_6 - Region 2 - NRPS-like

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Location: 382,876 - 446,643 nt. (total: 63,768 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 382875 - 446643:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1596210

AMP-binding (32..370): X

nrpys: Gly, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGASGGRFLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_6 - Region 3 - T1PKS

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Location: 1,455,895 - 1,521,444 nt. (total: 65,550 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 3, location 1455894 - 1521444:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1577527

PKS_AT (934..1231): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Propionyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 92.3
inactive: 56.5
Methylmalonyl-CoA: 52.1
Methoxymalonyl-CoA: 50.6
fatty_acid: 30.9
Isobutyryl-CoA: 29.1
Propionyl-CoA: 28.6
Ethylmalonyl-CoA: 24.7
Benzoyl-CoA: 24.7
CHC-CoA: 19.9
2-Methylbutyryl-CoA: 15.3
Acetyl-CoA: 12.7
trans-1,2-CPDA: 9.4
3-Methylbutyryl-CoA: 6.3

scaffold_7 - Region 1 - T1PKS

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Location: 1,094,686 - 1,161,118 nt. (total: 66,433 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 1094685 - 1161118:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1397166

PKS_AT (606..926): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 94.2
Methylmalonyl-CoA: 87.2
Methoxymalonyl-CoA: 85.6
Ethylmalonyl-CoA: 59.3
Isobutyryl-CoA: 49.9
trans-1,2-CPDA: 38.5
Benzoyl-CoA: 37.2
fatty_acid: 34.7
2-Methylbutyryl-CoA: 34.2
inactive: 32.6
Acetyl-CoA: 32.4
Propionyl-CoA: 29.4
CHC-CoA: 16.8
3-Methylbutyryl-CoA: 14.9

scaffold_7 - Region 2 - NRPS,T1PKS

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Location: 1,581,068 - 1,675,968 nt. (total: 94,901 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 1581067 - 1675968:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 1581067 - 1644685:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 1609480 - 1675968:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1422836

Search NORINE for peptide: strict or relaxed

AMP-binding (63..475): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: SISPDAVCIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

1660610

PKS_AT (899..1198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 100.7
inactive: 64.1
Methoxymalonyl-CoA: 53.4
Methylmalonyl-CoA: 53.0
Propionyl-CoA: 35.7
Ethylmalonyl-CoA: 16.2
Isobutyryl-CoA: 13.9
Benzoyl-CoA: 13.2
2-Methylbutyryl-CoA: 12.9
CHC-CoA: 12.1
fatty_acid: 10.5
Acetyl-CoA: 8.8
3-Methylbutyryl-CoA: 8.8
trans-1,2-CPDA: 0.0

scaffold_8 - Region 1 - NRPS-like

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Location: 108,040 - 165,891 nt. (total: 57,852 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 108039 - 165891:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1578796

AMP-binding (64..341): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AISALTF--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_8 - Region 2 - T1PKS

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Location: 500,292 - 568,283 nt. (total: 67,992 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 500291 - 568283:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1441742

PKS_AT (600..913): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 70.5
Methoxymalonyl-CoA: 59.3
Malonyl-CoA: 33.7
Isobutyryl-CoA: 29.6
trans-1,2-CPDA: 29.4
Propionyl-CoA: 26.8
inactive: 13.8
fatty_acid: 11.6
Benzoyl-CoA: 9.2
2-Methylbutyryl-CoA: 0.6
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.4

scaffold_8 - Region 3 - NRPS-like

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Location: 1,182,406 - 1,247,220 nt. (total: 64,815 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1182405 - 1247220:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1642724

Search NORINE for peptide: strict or relaxed

AMP-binding (27..499): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (94% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_9 - Region 1 - NRPS-like

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Location: 128,642 - 191,824 nt. (total: 63,183 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

1579646

AMP-binding (30..434): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVYFVGGTCK
Nearest Stachelhaus code(s):: Trp DVYFVGGICK (65% 8� match); Trp or Pro DVYFVGGICK (65% 8� match)
Stachelhaus code match:: 90% (moderate)