Usnea florida ATCC18376 v1.0

Identified secondary metabolite regions using strictness 'relaxed'

scaffold_1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	terpene	87,435	118,996

scaffold_10

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	T1PKS	671,320	752,232	6-methylsalicyclic acid	Polyketide	100%

scaffold_101

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	T1PKS	2,063	67,632	cryptosporioptide B/cryptosporioptide A/cryptosporioptide C	Polyketide:Iterative type I polyketide	46%

scaffold_11

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	terpene	17,150	33,692	koraiol	Terpene	100%
Region 13.2	terpene	634,412	666,384

scaffold_12

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	T1PKS	569,102	637,321	byssochlamic acid	Polyketide:Iterative type I polyketide	25%
Region 24.2	fungal-RiPP-like	675,158	765,818

scaffold_13

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	fungal-RiPP-like,T1PKS	14,357	122,268
Region 35.2	fungal-RiPP-like	133,119	242,070
Region 35.3	terpene	577,748	610,078	clavaric acid	Terpene	100%
Region 35.4	NRPS,T1PKS	670,734	742,689	CJ-16,173/CJ-15,696/citridone B/citridone A/citridone B'	Polyketide+NRP	57%

scaffold_14

Region	Type	From	To
Region 46.1	fungal-RiPP-like	1	79,734
Region 46.2	T1PKS	622,643	690,772
Region 46.3	NRPS	707,834	750,444

scaffold_16

Region	Type	From	To	Most similar known cluster		Similarity
Region 68.1	T1PKS	200,124	265,554
Region 68.2	NRPS-like	286,324	349,341

scaffold_17

Region	Type	From	To	Most similar known cluster		Similarity
Region 79.1	T1PKS	113,020	176,168
Region 79.2	T1PKS	434,226	503,159

scaffold_19

Region	Type	From	To	Most similar known cluster		Similarity
Region 101.1	NRPS-like	244,929	303,721
Region 101.2	NRPS-like,T1PKS	414,796	533,402	usnic acid	Polyketide	40%

scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 112.1	T1PKS	56,319	111,702	grayanic acid	Polyketide	66%
Region 112.2	T1PKS	499,500	566,709
Region 112.3	NRPS	715,259	795,658	nanangelenin B/terreazepine	NRP	5%

scaffold_20

Region	Type	From	To
Region 113.1	NRPS-like	4,266	67,100
Region 113.2	T1PKS	224,364	292,641
Region 113.3	terpene	627,969	659,975

scaffold_22

Region	Type	From	To	Most similar known cluster		Similarity
Region 135.1	T1PKS,fungal-RiPP-like	122,482	266,458
Region 135.2	NRPS-like	337,126	400,345
Region 135.3	T1PKS	556,919	605,859	rufoschweinitzin	Polyketide	42%

scaffold_23

Region	Type	From	To	Most similar known cluster		Similarity
Region 146.1	fungal-RiPP-like	35,963	129,411
Region 146.2	T1PKS	261,873	328,242

scaffold_24

Region	Type	From	To	Most similar known cluster		Similarity
Region 157.1	T1PKS	478,537	548,099

scaffold_26

Region	Type	From	To	Most similar known cluster		Similarity
Region 179.1	NRPS-like	12,278	76,712

scaffold_28

Region	Type	From	To	Most similar known cluster		Similarity
Region 196.1	T1PKS	49,511	121,507
Region 196.2	T1PKS	368,367	436,717

scaffold_29

Region	Type	From	To	Most similar known cluster		Similarity
Region 197.1	NRPS	404,014	468,999	AbT1	NRP	100%

scaffold_3

Region	Type	From	To	Most similar known cluster		Similarity
Region 198.1	fungal-RiPP-like,terpene	532,730	625,114	shearinine D	Terpene	9%

scaffold_30

Region	Type	From	To	Most similar known cluster		Similarity
Region 199.1	fungal-RiPP-like	116,728	207,867
Region 199.2	T1PKS	408,060	475,330

scaffold_31

Region	Type	From	To	Most similar known cluster		Similarity
Region 200.1	T1PKS	179,470	247,005
Region 200.2	terpene	255,664	287,315	squalestatin S1	Terpene	40%

scaffold_32

Region	Type	From	To	Most similar known cluster		Similarity
Region 201.1	T1PKS	115,994	183,707

scaffold_34

Region	Type	From	To	Most similar known cluster		Similarity
Region 203.1	NRPS	37,172	100,372	astellolide A	Terpene	50%
Region 203.2	fungal-RiPP-like	205,407	296,417

scaffold_37

Region	Type	From	To	Most similar known cluster		Similarity
Region 206.1	NRPS-like	117,810	181,422
Region 206.2	T1PKS	266,037	334,301

scaffold_38

Region	Type	From	To	Most similar known cluster		Similarity
Region 207.1	T1PKS	175,706	244,041

scaffold_39

Region	Type	From	To	Most similar known cluster		Similarity
Region 208.1	fungal-RiPP-like,NRPS-like	106,513	227,535	cyclosporin	NRP	10%

scaffold_4

Region	Type	From	To	Most similar known cluster		Similarity
Region 209.1	NRPS-like	3,900	5,487
Region 209.2	NRPS-like	615,963	677,550

scaffold_40

Region	Type	From	To	Most similar known cluster		Similarity
Region 210.1	T1PKS	62,467	119,910
Region 210.2	NRPS,NRPS-like	163,251	276,504
Region 210.3	T1PKS	288,160	354,888	scytalone/T3HN	Polyketide	40%

scaffold_41

Region	Type	From	To	Most similar known cluster		Similarity
Region 211.1	NRPS,T1PKS	52,976	123,365

scaffold_42

Region	Type	From	To	Most similar known cluster		Similarity
Region 212.1	NRPS-like	380,545	421,418

scaffold_44

Region	Type	From	To	Most similar known cluster		Similarity
Region 214.1	NRPS-like,indole	290,249	352,216	ergotamine	Terpene	52%

scaffold_45

Region	Type	From	To	Most similar known cluster		Similarity
Region 215.1	T1PKS	175,925	243,048
Region 215.2	T1PKS	311,598	377,071	terreic acid	Polyketide	33%

scaffold_46

Region	Type	From	To	Most similar known cluster		Similarity
Region 216.1	T1PKS,NRPS-like	320,081	368,703

scaffold_47

Region	Type	From	To	Most similar known cluster		Similarity
Region 217.1	fungal-RiPP-like,T1PKS	1	95,068

scaffold_48

Region	Type	From	To	Most similar known cluster		Similarity
Region 218.1	fungal-RiPP-like,T1PKS	127,527	289,261	cryptosporioptide B/cryptosporioptide A/cryptosporioptide C	Polyketide:Iterative type I polyketide	7%

scaffold_5

Region	Type	From	To	Most similar known cluster		Similarity
Region 220.1	T1PKS	87,437	155,763	monascorubrin	Polyketide	100%
Region 220.2	NRPS-like	646,613	709,989	patulin	Polyketide:Iterative type I polyketide	13%

scaffold_50

Region	Type	From	To	Most similar known cluster		Similarity
Region 221.1	fungal-RiPP-like	158,126	249,134

scaffold_52

Region	Type	From	To	Most similar known cluster		Similarity
Region 223.1	fungal-RiPP	184,763	246,536	ochrindole A	Other	17%

scaffold_54

Region	Type	From	To	Most similar known cluster		Similarity
Region 225.1	T1PKS	37,580	100,686	UNII-YC2Q1O94PT	Polyketide	100%
Region 225.2	NRP-metallophore,NRPS	192,132	266,980

scaffold_55

Region	Type	From	To	Most similar known cluster		Similarity
Region 226.1	T1PKS	112,203	176,077
Region 226.2	T1PKS	242,954	308,611	TAN-1612/1-(2,3,5,10-tetrahydroxy-7-methoxy-4-oxo-1,2,3,4-tetrahydroanthracen-2-yl)pentane-2,4-dione/desmethyl TAN-1612	Polyketide:Iterative type I polyketide	80%

scaffold_56

Region	Type	From	To	Most similar known cluster		Similarity
Region 227.1	fungal-RiPP-like	170,880	262,112

scaffold_57

Region	Type	From	To	Most similar known cluster		Similarity
Region 228.1	NRPS-like	28,802	92,293

scaffold_58

Region	Type	From	To	Most similar known cluster		Similarity
Region 229.1	T1PKS	42,412	110,441

scaffold_6

Region	Type	From	To	Most similar known cluster		Similarity
Region 231.1	NRPS-like	530,968	594,390

scaffold_60

Region	Type	From	To	Most similar known cluster		Similarity
Region 232.1	T1PKS,fungal-RiPP-like,T3PKS	188,123	282,516

scaffold_62

Region	Type	From	To	Most similar known cluster		Similarity
Region 234.1	fungal-RiPP	105,773	154,915	phomopsin A/phomopsin B/phomopsin E	RiPP	14%

scaffold_63

Region	Type	From	To	Most similar known cluster		Similarity
Region 235.1	T3PKS	21,922	83,286
Region 235.2	T1PKS	150,876	218,069	ochratoxin A	NRP+Polyketide	100%

scaffold_64

Region	Type	From	To	Most similar known cluster		Similarity
Region 236.1	terpene,T1PKS,NRPS	52,539	204,028	monacolin K	Polyketide	11%

scaffold_65

Region	Type	From	To	Most similar known cluster		Similarity
Region 237.1	indole	198,601	229,341	biscognienyne B/eutypinic acid/compound 3/4-hydroxy-3-prenylbenzoic acid/prenylhydroquinone/compound 15/compound 8/compound 7/compound 6/compound 11/compound 12/pestalodiol E	Other	54%

scaffold_66

Region	Type	From	To	Most similar known cluster		Similarity
Region 238.1	NRPS-like,NRPS,T1PKS	103,536	186,039

scaffold_67

Region	Type	From	To	Most similar known cluster		Similarity
Region 239.1	fungal-RiPP-like	130,093	221,233

scaffold_68

Region	Type	From	To	Most similar known cluster		Similarity
Region 240.1	fungal-RiPP-like	70,893	168,486

scaffold_69

Region	Type	From	To	Most similar known cluster		Similarity
Region 241.1	T1PKS	177,303	224,401

scaffold_7

Region	Type	From	To	Most similar known cluster		Similarity
Region 242.1	T1PKS	211,693	279,606

scaffold_71

Region	Type	From	To	Most similar known cluster		Similarity
Region 244.1	T1PKS	150,191	216,758	1,3,6,8-tetrahydroxynaphthalene	Polyketide	100%

scaffold_75

Region	Type	From	To	Most similar known cluster		Similarity
Region 248.1	fungal-RiPP-like	6,040	96,776

scaffold_76

Region	Type	From	To	Most similar known cluster		Similarity
Region 249.1	T1PKS	1,580	69,599	sordarial	Polyketide	40%

scaffold_77

Region	Type	From	To	Most similar known cluster		Similarity
Region 250.1	NRPS,T1PKS	1	72,010	phyllostictine A/phyllostictine B	NRP+Polyketide	50%
Region 250.2	fungal-RiPP-like,NRPS-like	134,538	196,698

scaffold_83

Region	Type	From	To	Most similar known cluster		Similarity
Region 257.1	fungal-RiPP-like	1	59,669

scaffold_9

Region	Type	From	To	Most similar known cluster		Similarity
Region 264.1	T1PKS,fungal-RiPP-like	292,597	463,049
Region 264.2	T1PKS	629,103	697,298

scaffold_90

Region	Type	From	To	Most similar known cluster		Similarity
Region 265.1	NRPS-like	94,644	139,108

scaffold_93

Region	Type	From	To	Most similar known cluster		Similarity
Region 268.1	T1PKS,NRPS-like	43,383	120,270

scaffold_94

Region	Type	From	To	Most similar known cluster		Similarity
Region 269.1	indole	68,379	100,343

scaffold_95

Region	Type	From	To	Most similar known cluster		Similarity
Region 270.1	T1PKS	1	79,207	trienylfuranol A/sporothriolide/dihydrosporothriolide/deoxysporothric acid/dihydroisosporothric acid/sporothric acid/isosporothric acid/sporochartine B	Polyketide	12%

scaffold_96

Region	Type	From	To	Most similar known cluster		Similarity
Region 271.1	T1PKS	1	65,855	zopfiellin	Polyketide	41%

scaffold_99

Region	Type	From	To	Most similar known cluster		Similarity
Region 274.1	terpene	14,792	45,916	nivalenol/deoxynivalenol/3-acetyldeoxynivalenol/15-acetyldeoxynivalenol/neosolaniol/calonectrin/apotrichodiol/isotrichotriol/15-decalonectrin/T-2 toxin/3-acetyl T-2 toxin/trichodiene	Terpene	8%

No secondary metabolite regions were found in these records:: scaffold_100; scaffold_102; scaffold_103; scaffold_104; scaffold_105; scaffold_106; scaffold_107; scaffold_108; scaffold_109; scaffold_110; scaffold_111; scaffold_112; scaffold_113; scaffold_114; scaffold_115; scaffold_116; scaffold_117; scaffold_118; scaffold_119; scaffold_120; scaffold_121; scaffold_122; scaffold_123; scaffold_124; scaffold_125; scaffold_126; scaffold_127; scaffold_128; scaffold_129; scaffold_130; scaffold_131; scaffold_132; scaffold_133; scaffold_134; scaffold_135; scaffold_136; scaffold_137; scaffold_138; scaffold_139; scaffold_140; scaffold_141; scaffold_142; scaffold_143; scaffold_144; scaffold_145; scaffold_146; scaffold_147; scaffold_148; scaffold_149; scaffold_15; scaffold_150; scaffold_151; scaffold_152; scaffold_153; scaffold_154; scaffold_155; scaffold_156; scaffold_157; scaffold_158; scaffold_159; scaffold_160; scaffold_161; scaffold_162; scaffold_163; scaffold_164; scaffold_165; scaffold_166; scaffold_167; scaffold_168; scaffold_169; scaffold_170; scaffold_171; scaffold_172; scaffold_173; scaffold_174; scaffold_175; scaffold_176; scaffold_177; scaffold_178; scaffold_179; scaffold_18; scaffold_180; scaffold_181; scaffold_182; scaffold_183; scaffold_184; scaffold_185; scaffold_186; scaffold_187; scaffold_188; scaffold_189; scaffold_190; scaffold_191; scaffold_192; scaffold_193; scaffold_194; scaffold_195; scaffold_196; scaffold_197; scaffold_198; scaffold_199; scaffold_200; scaffold_201; scaffold_202; scaffold_203; scaffold_204; scaffold_205; scaffold_206; scaffold_207; scaffold_208; scaffold_209; scaffold_21; scaffold_210; scaffold_211; scaffold_212; scaffold_213; scaffold_214; scaffold_215; scaffold_216; scaffold_217; scaffold_218; scaffold_219; scaffold_220; scaffold_221; scaffold_222; scaffold_223; scaffold_224; scaffold_225; scaffold_226; scaffold_227; scaffold_228; scaffold_229; scaffold_230; scaffold_231; scaffold_232; scaffold_233; scaffold_234; scaffold_235; scaffold_236; scaffold_237; scaffold_238; scaffold_239; scaffold_240; scaffold_241; scaffold_242; scaffold_243; scaffold_244; scaffold_245; scaffold_246; scaffold_247; scaffold_248; scaffold_249; scaffold_25; scaffold_250; scaffold_251; scaffold_252; scaffold_253; scaffold_254; scaffold_255; scaffold_256; scaffold_257; scaffold_258; scaffold_259; scaffold_260; scaffold_261; scaffold_262; scaffold_263; scaffold_264; scaffold_265; scaffold_266; scaffold_267; scaffold_268; scaffold_269; scaffold_27; scaffold_270; scaffold_271; scaffold_272; scaffold_273; scaffold_274; scaffold_33; scaffold_35; scaffold_36; scaffold_43; scaffold_49; scaffold_51; scaffold_53; scaffold_59; scaffold_61; scaffold_70; scaffold_72; scaffold_73; scaffold_74; scaffold_78; scaffold_79; scaffold_8; scaffold_80; scaffold_81; scaffold_82; scaffold_84; scaffold_85; scaffold_86; scaffold_87; scaffold_88; scaffold_89; scaffold_91; scaffold_92; scaffold_97; scaffold_98

Compact view

scaffold_1 - Region 1 - terpene

Download region GenBank file

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Location: 87,435 - 118,996 nt. (total: 31,562 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_10 - Region 1 - T1PKS

Download region GenBank file

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Location: 671,320 - 752,232 nt. (total: 80,913 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 671319 - 752232:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 886551 + 886548

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

886548

PKS_AT (914..1204): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.3
Methoxymalonyl-CoA: 62.1
Methylmalonyl-CoA: 54.1
inactive: 51.9
Propionyl-CoA: 32.2
2-Methylbutyryl-CoA: 27.4
Isobutyryl-CoA: 24.3
3-Methylbutyryl-CoA: 20.2
Ethylmalonyl-CoA: 19.9
Benzoyl-CoA: 16.5
fatty_acid: 15.1
CHC-CoA: 15.0
Acetyl-CoA: 13.8
trans-1,2-CPDA: 9.1

886551

PKS_AT (547..837): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 94.4
Methoxymalonyl-CoA: 81.8
Ethylmalonyl-CoA: 62.4
Isobutyryl-CoA: 50.5
Malonyl-CoA: 48.6
trans-1,2-CPDA: 41.5
Propionyl-CoA: 40.6
Acetyl-CoA: 34.1
2-Methylbutyryl-CoA: 31.1
Benzoyl-CoA: 23.2
fatty_acid: 20.5
3-Methylbutyryl-CoA: 20.5
CHC-CoA: 12.9
inactive: 0.0

scaffold_101 - Region 1 - T1PKS

Download region GenBank file

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Location: 2,063 - 67,632 nt. (total: 65,570 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 2062 - 67632:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

877001

AMP-binding (320..709): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VALTRSSSVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

895525

PKS_AT (935..1234): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 95.2
Methylmalonyl-CoA: 55.2
inactive: 52.3
Methoxymalonyl-CoA: 50.7
Propionyl-CoA: 32.3
fatty_acid: 28.5
Isobutyryl-CoA: 26.7
CHC-CoA: 24.8
2-Methylbutyryl-CoA: 22.5
Benzoyl-CoA: 18.2
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 12.4
3-Methylbutyryl-CoA: 10.9
trans-1,2-CPDA: 8.0

scaffold_11 - Region 1 - terpene

Download region GenBank file

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Location: 17,150 - 33,692 nt. (total: 16,543 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

scaffold_11 - Region 2 - terpene

Download region GenBank file

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Location: 634,412 - 666,384 nt. (total: 31,973 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_12 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 569,102 - 637,321 nt. (total: 68,220 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 569101 - 637321:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

802077

PKS_AT (596..916): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; 2-Methylbutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 81.8
Methoxymalonyl-CoA: 72.9
Malonyl-CoA: 66.2
Isobutyryl-CoA: 61.0
Ethylmalonyl-CoA: 44.2
Propionyl-CoA: 43.9
2-Methylbutyryl-CoA: 38.3
Benzoyl-CoA: 37.7
inactive: 23.2
fatty_acid: 18.3
3-Methylbutyryl-CoA: 16.4
trans-1,2-CPDA: 14.9
CHC-CoA: 11.4
Acetyl-CoA: 0.0

802160

AMP-binding (13..406): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GLGTRCAHQK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

887020

AMP-binding (88..556): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ERESIA--DK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_12 - Region 2 - fungal-RiPP-like

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Location: 675,158 - 765,818 nt. (total: 90,661 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_13 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 14,357 - 122,268 nt. (total: 107,912 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 14356 - 122268:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 59108 - 122268:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

929272

PKS_AT (898..1199): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 108.2
inactive: 56.7
Methoxymalonyl-CoA: 46.2
Methylmalonyl-CoA: 45.0
Isobutyryl-CoA: 19.6
Benzoyl-CoA: 18.3
Propionyl-CoA: 16.6
Acetyl-CoA: 14.7
CHC-CoA: 13.5
fatty_acid: 12.5
2-Methylbutyryl-CoA: 10.2
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_13 - Region 2 - fungal-RiPP-like

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Location: 133,119 - 242,070 nt. (total: 108,952 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_13 - Region 3 - terpene

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Location: 577,748 - 610,078 nt. (total: 32,331 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_13 - Region 4 - NRPS,T1PKS

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Location: 670,734 - 742,689 nt. (total: 71,956 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 8, location 670733 - 742689:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

946619

AMP-binding (128..535): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ELTVIVEVWK
Nearest Stachelhaus code(s):: Ala DLWNIGEVWK (32% 8� match); Asn DLTKIGEVGK (29% 8� match)
Stachelhaus code match:: 60% (weak)

897565

Search NORINE for peptide: strict or relaxed

PKS_AT (550..864): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 96.5
Methoxymalonyl-CoA: 84.4
Ethylmalonyl-CoA: 65.6
Malonyl-CoA: 61.0
Isobutyryl-CoA: 50.5
Benzoyl-CoA: 46.8
Propionyl-CoA: 40.6
3-Methylbutyryl-CoA: 26.1
trans-1,2-CPDA: 25.1
2-Methylbutyryl-CoA: 22.6
CHC-CoA: 22.5
fatty_acid: 21.7
Acetyl-CoA: 19.2
inactive: 18.6

AMP-binding (3029..3429): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMAICGCCAK
Nearest Stachelhaus code(s):: Phe DMAICGCCAK (88% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_14 - Region 1 - fungal-RiPP-like

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Location: 1 - 79,734 nt. (total: 79,734 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_14 - Region 2 - T1PKS

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Location: 622,643 - 690,772 nt. (total: 68,130 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 622642 - 690772:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

971309

PKS_AT (916..1215): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: inactive

Prediction, score:

inactive: 49.8
Malonyl-CoA: 48.6
Methoxymalonyl-CoA: 28.4
Methylmalonyl-CoA: 22.0
CHC-CoA: 11.6
Benzoyl-CoA: 11.4
Propionyl-CoA: 11.2
Ethylmalonyl-CoA: 11.0
fatty_acid: 9.4
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_14 - Region 3 - NRPS

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Location: 707,834 - 750,444 nt. (total: 42,611 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 3, location 707833 - 750444:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - D-Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

819795

AMP-binding (62..504): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -ALQNLFVSK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

819801

Search NORINE for peptide: strict or relaxed

AMP-binding (586..986): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DADAIGASIK
Nearest Stachelhaus code(s):: Tyr DAMSIGAVIK (47% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1676..2074): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DFAFIGIVMK
Nearest Stachelhaus code(s):: Ala DVMFIGIVAK (50% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_16 - Region 1 - T1PKS

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Location: 200,124 - 265,554 nt. (total: 65,431 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 200123 - 265554:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

803676

PKS_AT (556..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 59.5
inactive: 52.2
Methoxymalonyl-CoA: 38.4
Methylmalonyl-CoA: 24.1
Benzoyl-CoA: 16.5
Isobutyryl-CoA: 11.4
CHC-CoA: 10.0
fatty_acid: 9.7
3-Methylbutyryl-CoA: 9.6
Propionyl-CoA: 9.5
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_16 - Region 2 - NRPS-like

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Location: 286,324 - 349,341 nt. (total: 63,018 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

NRPS/PKS monomer predictions

820071

AMP-binding (1..394): X

nrpys: Tyr, Phe

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DARVVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (65% 8� match); Phe DAWTVAAVCK (62% 8� match)
Stachelhaus code match:: 80% (moderate)

845090

AMP-binding (42..474): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AMTCTAVGPK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_17 - Region 1 - T1PKS

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Location: 113,020 - 176,168 nt. (total: 63,149 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 113019 - 176168:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

804000

PKS_AT (547..855): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 86.7
Methoxymalonyl-CoA: 81.5
Malonyl-CoA: 71.4
Isobutyryl-CoA: 54.4
Propionyl-CoA: 45.7
2-Methylbutyryl-CoA: 43.0
Ethylmalonyl-CoA: 34.6
trans-1,2-CPDA: 33.7
Benzoyl-CoA: 32.8
inactive: 30.9
CHC-CoA: 22.0
fatty_acid: 20.7
3-Methylbutyryl-CoA: 11.8
Acetyl-CoA: 0.0

scaffold_17 - Region 2 - T1PKS

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Location: 434,226 - 503,159 nt. (total: 68,934 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 434225 - 503159:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

804034

PKS_AT (417..743): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.8
Methoxymalonyl-CoA: 82.6
Methylmalonyl-CoA: 80.7
Ethylmalonyl-CoA: 46.5
Isobutyryl-CoA: 44.7
Propionyl-CoA: 34.3
Benzoyl-CoA: 25.5
fatty_acid: 24.2
inactive: 22.5
CHC-CoA: 17.9
trans-1,2-CPDA: 17.8
2-Methylbutyryl-CoA: 17.0
3-Methylbutyryl-CoA: 12.3
Acetyl-CoA: 1.9

scaffold_19 - Region 1 - NRPS-like

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Location: 244,929 - 303,721 nt. (total: 58,793 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 244928 - 303721:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

804615

AMP-binding (43..334): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GYWHISLWGK
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (29% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_19 - Region 2 - NRPS-like,T1PKS

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Location: 414,796 - 533,402 nt. (total: 118,607 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 414795 - 533402:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 414795 - 477579:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 465340 - 533402:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

888451

AMP-binding (30..437): X

nrpys: Ppa

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VATFIGGAGK
Nearest Stachelhaus code(s):: Ppa VATFIGGAGK (91% 8� match)
Stachelhaus code match:: 100% (strong)

179021

PKS_AT (908..1198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 56.0
Methoxymalonyl-CoA: 53.5
Methylmalonyl-CoA: 40.6
inactive: 29.0
Propionyl-CoA: 21.8
CHC-CoA: 21.5
Ethylmalonyl-CoA: 20.5
trans-1,2-CPDA: 18.5
2-Methylbutyryl-CoA: 13.2
Isobutyryl-CoA: 12.0
3-Methylbutyryl-CoA: 11.2
Benzoyl-CoA: 10.9
fatty_acid: 0.0
Acetyl-CoA: 0.0

scaffold_2 - Region 1 - T1PKS

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Location: 56,319 - 111,702 nt. (total: 55,384 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 56318 - 111702:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

884194

PKS_AT (895..1197): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 82.3
Methoxymalonyl-CoA: 58.0
Methylmalonyl-CoA: 53.6
inactive: 29.8
Propionyl-CoA: 28.0
Ethylmalonyl-CoA: 24.9
2-Methylbutyryl-CoA: 22.9
CHC-CoA: 20.8
Acetyl-CoA: 19.0
Benzoyl-CoA: 16.3
Isobutyryl-CoA: 13.5
3-Methylbutyryl-CoA: 12.3
trans-1,2-CPDA: 11.5
fatty_acid: 9.4

scaffold_2 - Region 2 - T1PKS

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Location: 499,500 - 566,709 nt. (total: 67,210 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 499499 - 566709:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

906529

PKS_AT (591..901): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 86.3
Malonyl-CoA: 80.8
Methoxymalonyl-CoA: 76.1
Isobutyryl-CoA: 57.4
Ethylmalonyl-CoA: 53.0
Benzoyl-CoA: 49.7
Propionyl-CoA: 42.1
2-Methylbutyryl-CoA: 34.9
trans-1,2-CPDA: 27.0
fatty_acid: 17.1
CHC-CoA: 16.0
Acetyl-CoA: 15.3
inactive: 12.9
3-Methylbutyryl-CoA: 0.0

scaffold_2 - Region 3 - NRPS

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Location: 715,259 - 795,658 nt. (total: 80,400 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 3, location 715258 - 795658:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Phe - X - D-Leu - Leu - D-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

193529

Search NORINE for peptide: strict or relaxed

AMP-binding (57..454): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQVLVGVAK
Nearest Stachelhaus code(s):: Phe DAFVLAGVAK (62% 8� match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1600..1997): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNEDAGQINK
Nearest Stachelhaus code(s):: Arg DAEDVGVIGK (53% 8� match); OH-Orn DMENLGLINK (47% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (2682..3079): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAVLVGAVVK
Nearest Stachelhaus code(s):: Leu DALLVGAVVK (62% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4219..4622): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMLVGAVIK
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (56% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (5310..5710): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVMFVGGVIK
Nearest Stachelhaus code(s):: Val DAMFVGGVFK (68% 8� match); Ant DVMFVGEVAK (62% 8� match)
Stachelhaus code match:: 80% (moderate)

scaffold_20 - Region 1 - NRPS-like

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Location: 4,266 - 67,100 nt. (total: 62,835 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 4265 - 67100:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

804970

AMP-binding (34..448): X

nrpys: Ppa

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VATFIGGAGK
Nearest Stachelhaus code(s):: Ppa VATFIGGAGK (91% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_20 - Region 2 - T1PKS

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Location: 224,364 - 292,641 nt. (total: 68,278 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 224363 - 292641:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

898139

PKS_AT (610..930): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 80.7
Malonyl-CoA: 77.7
Methoxymalonyl-CoA: 69.2
Isobutyryl-CoA: 51.6
Ethylmalonyl-CoA: 49.0
Propionyl-CoA: 45.7
fatty_acid: 38.7
2-Methylbutyryl-CoA: 30.2
inactive: 23.2
trans-1,2-CPDA: 20.9
Benzoyl-CoA: 15.8
CHC-CoA: 12.1
Acetyl-CoA: 9.8
3-Methylbutyryl-CoA: 0.0

scaffold_20 - Region 3 - terpene

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Location: 627,969 - 659,975 nt. (total: 32,007 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_22 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 122,482 - 266,458 nt. (total: 143,977 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328

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Predicted core structure(s)

For candidate cluster 1, location 122481 - 266458:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 805551 + 219482

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 122481 - 195162:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 805551 + 219482

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

219482

PKS_AT (891..1191): mal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 58.3%; Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 110.2
inactive: 69.1
Methylmalonyl-CoA: 62.7
Methoxymalonyl-CoA: 57.5
Isobutyryl-CoA: 36.6
Ethylmalonyl-CoA: 29.2
Acetyl-CoA: 26.9
Propionyl-CoA: 21.1
3-Methylbutyryl-CoA: 20.8
Benzoyl-CoA: 19.8
CHC-CoA: 13.1
fatty_acid: 12.3
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 6.9

805551

PKS_AT (565..880): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Propionyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 84.6
Malonyl-CoA: 84.6
Methylmalonyl-CoA: 79.5
Isobutyryl-CoA: 53.8
trans-1,2-CPDA: 42.5
Propionyl-CoA: 28.9
Benzoyl-CoA: 27.8
CHC-CoA: 26.8
inactive: 23.3
fatty_acid: 18.0
Acetyl-CoA: 14.4
3-Methylbutyryl-CoA: 13.5
2-Methylbutyryl-CoA: 13.3
Ethylmalonyl-CoA: -3.7

scaffold_22 - Region 2 - NRPS-like

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Location: 337,126 - 400,345 nt. (total: 63,220 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 4, location 337125 - 400345:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

880594

AMP-binding (45..364): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AACAIALLPK
Nearest Stachelhaus code(s):: aza-bTyr DACELALLAK (29% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_22 - Region 3 - T1PKS

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Location: 556,919 - 605,859 nt. (total: 48,941 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 5, location 556918 - 605859:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

972629

PKS_AT (896..1200): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 106.5
inactive: 65.8
Methylmalonyl-CoA: 52.3
Methoxymalonyl-CoA: 45.0
Propionyl-CoA: 30.0
Benzoyl-CoA: 22.9
2-Methylbutyryl-CoA: 19.3
Isobutyryl-CoA: 18.8
Ethylmalonyl-CoA: 17.9
fatty_acid: 17.3
CHC-CoA: 15.8
Acetyl-CoA: 11.2
trans-1,2-CPDA: 9.4
3-Methylbutyryl-CoA: 3.2

scaffold_23 - Region 1 - fungal-RiPP-like

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Location: 35,963 - 129,411 nt. (total: 93,449 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_23 - Region 2 - T1PKS

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Location: 261,873 - 328,242 nt. (total: 66,370 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 261872 - 328242:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

821296

PKS_AT (766..1066): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.1
Methylmalonyl-CoA: 47.7
Methoxymalonyl-CoA: 46.6
inactive: 34.5
Isobutyryl-CoA: 29.9
Propionyl-CoA: 25.5
CHC-CoA: 24.4
Ethylmalonyl-CoA: 23.9
Acetyl-CoA: 21.6
Benzoyl-CoA: 21.0
3-Methylbutyryl-CoA: 18.0
trans-1,2-CPDA: 17.0
fatty_acid: 13.3
2-Methylbutyryl-CoA: -2.7

scaffold_24 - Region 1 - T1PKS

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Location: 478,537 - 548,099 nt. (total: 69,563 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 478536 - 548099:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

889366

PKS_AT (77..357): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 2.2
Malonyl-CoA: 0.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Methoxymalonyl-CoA: -0.2
Isobutyryl-CoA: -1.2

821518

PKS_AT (554..766): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 52.4
Methylmalonyl-CoA: 40.8
Malonyl-CoA: 34.6
Ethylmalonyl-CoA: 17.4
Isobutyryl-CoA: 11.3
inactive: 9.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_26 - Region 1 - NRPS-like

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Location: 12,278 - 76,712 nt. (total: 64,435 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 12277 - 76712:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

889554

Search NORINE for peptide: strict or relaxed

AMP-binding (30..507): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (94% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_28 - Region 1 - T1PKS

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Location: 49,511 - 121,507 nt. (total: 71,997 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 49510 - 121507:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 889859 + 270683

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

270683

PKS_AT (899..1198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 94.7
Methoxymalonyl-CoA: 53.5
Methylmalonyl-CoA: 53.4
inactive: 45.1
Propionyl-CoA: 23.5
Benzoyl-CoA: 22.6
Ethylmalonyl-CoA: 21.0
Isobutyryl-CoA: 18.0
CHC-CoA: 17.6
2-Methylbutyryl-CoA: 16.9
trans-1,2-CPDA: 15.6
Acetyl-CoA: 11.1
3-Methylbutyryl-CoA: 8.9
fatty_acid: 0.0

889859

PKS_AT (585..901): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 76.5
Methoxymalonyl-CoA: 71.5
Ethylmalonyl-CoA: 48.9
Isobutyryl-CoA: 48.7
Malonyl-CoA: 41.2
trans-1,2-CPDA: 22.7
Propionyl-CoA: 22.3
inactive: 17.9
2-Methylbutyryl-CoA: 16.1
fatty_acid: 12.9
Benzoyl-CoA: 11.8
CHC-CoA: 10.1
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_28 - Region 2 - T1PKS

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Location: 368,367 - 436,717 nt. (total: 68,351 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 368366 - 436717:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

909285

PKS_AT (565..802): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 11.6
Methylmalonyl-CoA: 10.1
Methoxymalonyl-CoA: 9.6
Malonyl-CoA: 8.3
trans-1,2-CPDA: 1.8
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_29 - Region 1 - NRPS

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Location: 404,014 - 468,999 nt. (total: 64,986 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS monomer predictions

950487

AMP-binding (797..1202): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWKLAAGFK
Nearest Stachelhaus code(s):: Val DAWMLAATLK (71% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_3 - Region 1 - fungal-RiPP-like,terpene

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Location: 532,730 - 625,114 nt. (total: 92,385 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_30 - Region 1 - fungal-RiPP-like

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Location: 116,728 - 207,867 nt. (total: 91,140 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_30 - Region 2 - T1PKS

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Location: 408,060 - 475,330 nt. (total: 67,271 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 408059 - 475330:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

807107

PKS_AT (351..672): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 69.9
Methoxymalonyl-CoA: 68.5
Malonyl-CoA: 63.6
Isobutyryl-CoA: 56.3
Ethylmalonyl-CoA: 44.3
Benzoyl-CoA: 18.9
Propionyl-CoA: 18.2
trans-1,2-CPDA: 14.4
fatty_acid: 8.8
CHC-CoA: 7.1
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -3.3

scaffold_31 - Region 1 - T1PKS

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Location: 179,470 - 247,005 nt. (total: 67,536 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 179469 - 247005:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

933683

PKS_AT (533..834): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Ethylmalonyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 56.6
Methoxymalonyl-CoA: 47.7
Isobutyryl-CoA: 37.9
Ethylmalonyl-CoA: 35.5
Malonyl-CoA: 23.3
Benzoyl-CoA: 21.8
Propionyl-CoA: 19.2
CHC-CoA: 17.9
2-Methylbutyryl-CoA: 11.5
fatty_acid: 9.9
3-Methylbutyryl-CoA: 9.2
inactive: 9.1
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0

scaffold_31 - Region 2 - terpene

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Location: 255,664 - 287,315 nt. (total: 31,652 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_32 - Region 1 - T1PKS

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Location: 115,994 - 183,707 nt. (total: 67,714 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 115993 - 183707:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

807691

PKS_AT (547..862): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 66.7%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 84.8
Methoxymalonyl-CoA: 83.1
Malonyl-CoA: 81.2
Isobutyryl-CoA: 53.4
Ethylmalonyl-CoA: 49.3
2-Methylbutyryl-CoA: 35.4
Propionyl-CoA: 32.8
trans-1,2-CPDA: 27.6
Benzoyl-CoA: 25.7
inactive: 23.6
Acetyl-CoA: 17.6
3-Methylbutyryl-CoA: 16.0
fatty_acid: 14.3
CHC-CoA: 13.1

scaffold_34 - Region 1 - NRPS

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Location: 37,172 - 100,372 nt. (total: 63,201 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 37171 - 100372:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

808082

AMP-binding (23..424): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLYTAALGPK
Nearest Stachelhaus code(s):: Ala DLYNNALTYK (47% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_34 - Region 2 - fungal-RiPP-like

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Location: 205,407 - 296,417 nt. (total: 91,011 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_37 - Region 1 - NRPS-like

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Location: 117,810 - 181,422 nt. (total: 63,613 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 117809 - 181422:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

808839

AMP-binding (37..343): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---FEGVL-K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_37 - Region 2 - T1PKS

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Location: 266,037 - 334,301 nt. (total: 68,265 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 266036 - 334301:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

912893

PKS_AT (579..886): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 97.5
Methoxymalonyl-CoA: 91.3
Malonyl-CoA: 77.5
Ethylmalonyl-CoA: 63.5
Isobutyryl-CoA: 59.3
Propionyl-CoA: 34.8
trans-1,2-CPDA: 33.0
Benzoyl-CoA: 31.7
inactive: 25.5
CHC-CoA: 18.9
fatty_acid: 18.2
2-Methylbutyryl-CoA: 17.6
3-Methylbutyryl-CoA: 9.3
Acetyl-CoA: 7.1

scaffold_38 - Region 1 - T1PKS

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Location: 175,706 - 244,041 nt. (total: 68,336 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomer predictions

881679

PKS_AT (450..759): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.7
Methoxymalonyl-CoA: 62.5
Isobutyryl-CoA: 57.6
Malonyl-CoA: 49.6
Propionyl-CoA: 40.8
Ethylmalonyl-CoA: 35.6
Benzoyl-CoA: 23.2
trans-1,2-CPDA: 15.1
fatty_acid: 13.0
2-Methylbutyryl-CoA: 10.4
3-Methylbutyryl-CoA: 9.1
inactive: 9.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_39 - Region 1 - NRPS-like,fungal-RiPP-like

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Location: 106,513 - 227,535 nt. (total: 121,023 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 106512 - 227535:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 164186 - 227535:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

891408

AMP-binding (46..366): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GGAFLAFAIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_4 - Region 1 - NRPS-like

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Location: 3,900 - 5,487 nt. (total: 1,588 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 3899 - 5487:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

959286

AMP-binding (0..322): X

nrpys: Phe, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARGTVTRLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_4 - Region 2 - NRPS-like

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Location: 615,963 - 677,550 nt. (total: 61,588 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 615962 - 677550:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

959415

AMP-binding (0..322): X

nrpys: Phe, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARGTVTRLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_40 - Region 1 - T1PKS

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Location: 62,467 - 119,910 nt. (total: 57,444 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_40 - Region 2 - NRPS,NRPS-like

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Location: 163,251 - 276,504 nt. (total: 113,254 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 163250 - 276504:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X - Ala - X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 163250 - 242557:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X - Ala - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 213146 - 276504:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

891507

Search NORINE for peptide: strict or relaxed

AMP-binding (501..898): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNLSTFKK
Nearest Stachelhaus code(s):: Orn DVSELGTINK (41% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1581..1978): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLCVAWVAK
Nearest Stachelhaus code(s):: Phe or Tyr DAACVAGVAK (47% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2664..3049): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DTICCC--FK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (3727..4136): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFCGVAIK
Nearest Stachelhaus code(s):: Abu DAWFHAVAYK (38% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (4835..5237): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DISHVGIQGK
Nearest Stachelhaus code(s):: Hpg DIWHVGAITK (41% 8� match)
Stachelhaus code match:: 60% (weak)

823561

AMP-binding (20..339): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AGAFCAY--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_40 - Region 3 - T1PKS

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Location: 288,160 - 354,888 nt. (total: 66,729 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 5, location 288159 - 354888:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

891540

PKS_AT (915..1212): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 130.3
inactive: 75.1
Methoxymalonyl-CoA: 59.1
Methylmalonyl-CoA: 56.8
Propionyl-CoA: 33.5
2-Methylbutyryl-CoA: 19.7
Isobutyryl-CoA: 18.5
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 15.7
fatty_acid: 14.5
Benzoyl-CoA: 13.1
CHC-CoA: 11.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_41 - Region 1 - NRPS,T1PKS

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Location: 52,976 - 123,365 nt. (total: 70,390 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 52975 - 123365:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

952558

Search NORINE for peptide: strict or relaxed

PKS_AT (505..815): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; inactive: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 60.9
Methoxymalonyl-CoA: 59.5
Isobutyryl-CoA: 55.2
3-Methylbutyryl-CoA: 29.7
Benzoyl-CoA: 27.6
inactive: 27.2
Ethylmalonyl-CoA: 25.9
Acetyl-CoA: 23.0
Propionyl-CoA: 20.7
Malonyl-CoA: 15.3
fatty_acid: 8.8
2-Methylbutyryl-CoA: 0.7
CHC-CoA: 0.6
trans-1,2-CPDA: -0.1

AMP-binding (2792..3204): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLVYFATVQK
Nearest Stachelhaus code(s):: Gly DLVYFATVQK (82% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_42 - Region 1 - NRPS-like

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Location: 380,545 - 421,418 nt. (total: 40,874 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 380544 - 421418:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

823854

AMP-binding (259..662): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVIIAGIICK
Nearest Stachelhaus code(s):: Gln DVQIVGGIHK (56% 8� match); Pro DMYFAGLICK (50% 8� match); Trp or Pro DVYFVGGICK (47% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_44 - Region 1 - NRPS-like,indole

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Location: 290,249 - 352,216 nt. (total: 61,968 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
indole: (indsynth or dmat or indole_PTase)

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Predicted core structure(s)

For candidate cluster 1, location 290248 - 352216:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

914934

AMP-binding (38..439): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVGLLVK
Nearest Stachelhaus code(s):: D-Lya DVFSVGLVMK (65% 8� match)
Stachelhaus code match:: 80% (moderate)

scaffold_45 - Region 1 - T1PKS

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Location: 175,925 - 243,048 nt. (total: 67,124 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 175924 - 243048:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

965256

PKS_AT (622..930): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 88.0
Methoxymalonyl-CoA: 84.0
Malonyl-CoA: 83.8
Isobutyryl-CoA: 77.6
Ethylmalonyl-CoA: 52.3
Benzoyl-CoA: 50.0
Propionyl-CoA: 39.3
trans-1,2-CPDA: 36.1
inactive: 31.0
3-Methylbutyryl-CoA: 29.2
fatty_acid: 22.4
Acetyl-CoA: 21.9
CHC-CoA: 10.2
2-Methylbutyryl-CoA: 7.6

scaffold_45 - Region 2 - T1PKS

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Location: 311,598 - 377,071 nt. (total: 65,474 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 311597 - 377071:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

965286

PKS_AT (563..855): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 93.9
Methoxymalonyl-CoA: 73.1
Ethylmalonyl-CoA: 69.2
Isobutyryl-CoA: 67.7
Malonyl-CoA: 57.1
Propionyl-CoA: 37.7
Acetyl-CoA: 32.3
2-Methylbutyryl-CoA: 31.2
Benzoyl-CoA: 30.2
fatty_acid: 28.9
3-Methylbutyryl-CoA: 21.6
trans-1,2-CPDA: 20.0
CHC-CoA: 16.1
inactive: -2.0

scaffold_46 - Region 1 - NRPS-like,T1PKS

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Location: 320,081 - 368,703 nt. (total: 48,623 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 320080 - 368703:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 328773 - 368703:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

892226

PKS_AT (892..1182): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 67.1
inactive: 46.8
Methoxymalonyl-CoA: 45.0
Methylmalonyl-CoA: 29.3
Ethylmalonyl-CoA: 16.7
CHC-CoA: 14.6
Propionyl-CoA: 13.7
fatty_acid: 13.2
2-Methylbutyryl-CoA: 11.9
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

810298

AMP-binding (38..369): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GFHVAGNPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_47 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 1 - 95,068 nt. (total: 95,068 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 95068:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 26794 - 95068:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

953410

PKS_AT (573..890): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 106.0
Methoxymalonyl-CoA: 83.5
Methylmalonyl-CoA: 83.2
Isobutyryl-CoA: 65.8
Ethylmalonyl-CoA: 51.4
inactive: 48.2
3-Methylbutyryl-CoA: 42.6
Propionyl-CoA: 39.9
2-Methylbutyryl-CoA: 39.8
Benzoyl-CoA: 36.4
CHC-CoA: 27.0
trans-1,2-CPDA: 25.4
Acetyl-CoA: 23.7
fatty_acid: 18.7

scaffold_48 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 127,527 - 289,261 nt. (total: 161,735 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 127526 - 289261:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 222470 - 289261:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

463564

PKS_AT (912..1209): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 102.3
inactive: 66.9
Methoxymalonyl-CoA: 40.8
Methylmalonyl-CoA: 33.6
Propionyl-CoA: 31.9
fatty_acid: 12.0
2-Methylbutyryl-CoA: 11.7
Benzoyl-CoA: 10.7
Isobutyryl-CoA: 9.6
Ethylmalonyl-CoA: 9.3
Acetyl-CoA: 8.1
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_5 - Region 1 - T1PKS

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Location: 87,437 - 155,763 nt. (total: 68,327 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 87436 - 155763:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

926105

AMP-binding (27..445): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -QALAA-RWK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

817430

PKS_AT (921..1217): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 59.6
Methylmalonyl-CoA: 50.5
inactive: 48.6
Ethylmalonyl-CoA: 31.1
Malonyl-CoA: 30.2
Propionyl-CoA: 29.4
CHC-CoA: 21.7
trans-1,2-CPDA: 18.6
Acetyl-CoA: 16.7
2-Methylbutyryl-CoA: 14.6
3-Methylbutyryl-CoA: 12.9
fatty_acid: 11.0
Isobutyryl-CoA: 10.7
Benzoyl-CoA: 9.0

scaffold_5 - Region 2 - NRPS-like

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Location: 646,613 - 709,989 nt. (total: 63,377 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 646612 - 709989:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

885173

AMP-binding (22..339): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TATAFA-NLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_50 - Region 1 - fungal-RiPP-like

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Location: 158,126 - 249,134 nt. (total: 91,009 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_52 - Region 1 - fungal-RiPP

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Location: 184,763 - 246,536 nt. (total: 61,774 nt) Show pHMM detection rules used

fungal-RiPP: ((pop or ustH) and (p450 or TP_methylase) or DUF3328 and (Peptidase_S41 or Peptidase_S28 or pop or ustH))

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scaffold_54 - Region 1 - T1PKS

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Location: 37,580 - 100,686 nt. (total: 63,107 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomer predictions

882464

PKS_AT (476..753): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 77.9
Methoxymalonyl-CoA: 74.4
Isobutyryl-CoA: 41.3
trans-1,2-CPDA: 33.6
2-Methylbutyryl-CoA: 31.7
Propionyl-CoA: 23.2
3-Methylbutyryl-CoA: 20.0
fatty_acid: 19.3
Benzoyl-CoA: 18.9
inactive: 13.6
CHC-CoA: 11.0
Acetyl-CoA: 10.0
Ethylmalonyl-CoA: 0.6
Malonyl-CoA: -2.0

scaffold_54 - Region 2 - NRP-metallophore,NRPS

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Location: 192,132 - 266,980 nt. (total: 74,849 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 2, location 192131 - 266980:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

917471

Search NORINE for peptide: strict or relaxed

AMP-binding (283..679): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQHTITVVK
Nearest Stachelhaus code(s):: Cys DVQHTITVVK (82% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1393..1797): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVASGGAIGK
Nearest Stachelhaus code(s):: E-ahmohOrn DVGGGGVIGK (71% 8� match); Arg DVESIGAIAK (53% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_55 - Region 1 - T1PKS

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Location: 112,203 - 176,077 nt. (total: 63,875 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 112202 - 176077:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

516973

PKS_AT (573..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Malonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.6
Methoxymalonyl-CoA: 63.6
Ethylmalonyl-CoA: 44.5
Benzoyl-CoA: 42.8
Isobutyryl-CoA: 40.5
Malonyl-CoA: 31.6
Propionyl-CoA: 30.3
trans-1,2-CPDA: 28.5
fatty_acid: 20.6
3-Methylbutyryl-CoA: 18.6
CHC-CoA: 13.4
Acetyl-CoA: 10.2
2-Methylbutyryl-CoA: 6.3
inactive: 0.0

scaffold_55 - Region 2 - T1PKS

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Location: 242,954 - 308,611 nt. (total: 65,658 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 242953 - 308611:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

917619

PKS_AT (926..1224): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: inactive

Prediction, score:

inactive: 58.5
Methylmalonyl-CoA: 47.9
Methoxymalonyl-CoA: 46.8
Malonyl-CoA: 40.3
Propionyl-CoA: 34.6
Isobutyryl-CoA: 20.2
Benzoyl-CoA: 17.1
2-Methylbutyryl-CoA: 17.0
Ethylmalonyl-CoA: 15.5
CHC-CoA: 11.3
fatty_acid: 9.4
3-Methylbutyryl-CoA: 8.7
Acetyl-CoA: 7.5
trans-1,2-CPDA: 0.0

scaffold_56 - Region 1 - fungal-RiPP-like

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Location: 170,880 - 262,112 nt. (total: 91,233 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_57 - Region 1 - NRPS-like

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Location: 28,802 - 92,293 nt. (total: 63,492 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 28801 - 92293:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

893238

AMP-binding (50..431): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NIVILAPTLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_58 - Region 1 - T1PKS

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Location: 42,412 - 110,441 nt. (total: 68,030 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 42411 - 110441:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

528127

PKS_AT (515..825): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 90.7
Methoxymalonyl-CoA: 81.0
Malonyl-CoA: 73.9
Ethylmalonyl-CoA: 60.0
Isobutyryl-CoA: 56.0
trans-1,2-CPDA: 34.6
Propionyl-CoA: 31.9
Benzoyl-CoA: 27.2
CHC-CoA: 14.8
2-Methylbutyryl-CoA: 12.4
3-Methylbutyryl-CoA: 12.0
fatty_acid: 5.9
inactive: 0.0
Acetyl-CoA: 0.0

scaffold_6 - Region 1 - NRPS-like

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Location: 530,968 - 594,390 nt. (total: 63,423 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 530967 - 594390:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

834887

AMP-binding (41..374): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGASGGRFLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_60 - Region 1 - T1PKS,T3PKS,fungal-RiPP-like

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Location: 188,123 - 282,516 nt. (total: 94,394 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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Predicted core structure(s)

For candidate cluster 1, location 188122 - 282516:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 188122 - 256153:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 196862 - 258221:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

900218

PKS_AT (509..801): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 66.2
Methoxymalonyl-CoA: 50.9
Propionyl-CoA: 41.4
Benzoyl-CoA: 18.2
trans-1,2-CPDA: 12.1
fatty_acid: 7.2
Malonyl-CoA: 4.1
Isobutyryl-CoA: 2.6
inactive: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.2
Ethylmalonyl-CoA: -2.9

scaffold_62 - Region 1 - fungal-RiPP

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Location: 105,773 - 154,915 nt. (total: 49,143 nt) Show pHMM detection rules used

fungal-RiPP: ((pop or ustH) and (p450 or TP_methylase) or DUF3328 and (Peptidase_S41 or Peptidase_S28 or pop or ustH))

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scaffold_63 - Region 1 - T3PKS

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Location: 21,922 - 83,286 nt. (total: 61,365 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_63 - Region 2 - T1PKS

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Location: 150,876 - 218,069 nt. (total: 67,194 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 150875 - 218069:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

900319

PKS_AT (890..1190): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.8
inactive: 60.1
Methylmalonyl-CoA: 57.7
Methoxymalonyl-CoA: 57.5
Propionyl-CoA: 30.3
Isobutyryl-CoA: 28.5
Benzoyl-CoA: 26.3
Ethylmalonyl-CoA: 23.4
Acetyl-CoA: 18.6
2-Methylbutyryl-CoA: 18.4
fatty_acid: 15.4
CHC-CoA: 15.4
trans-1,2-CPDA: 11.3
3-Methylbutyryl-CoA: 11.0

scaffold_64 - Region 1 - NRPS,T1PKS,terpene

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Location: 52,539 - 204,028 nt. (total: 151,490 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 52538 - 204028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Leu - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 115319 - 204028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Leu - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

919351

Search NORINE for peptide: strict or relaxed

AMP-binding (607..1006): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVCIIILTFK
Nearest Stachelhaus code(s):: Ala or Val DVFWIGGTFK (56% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1701..2098): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DSVDIGPPTK
Nearest Stachelhaus code(s):: Arg DVSDTGAPTK (65% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (3264..3670): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSDLSTVKK
Nearest Stachelhaus code(s):: Ser DVSHVSAVDK (50% 8� match); 4OH-Pro DVSSATTVCK (47% 8� match); Dpr DVRHLSMVEK (41% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (4336..4731): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSEIGSPTK
Nearest Stachelhaus code(s):: Arg DVSDTGAPTK (76% 8� match); Orn DVGEIGSVDK (38% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (7183..7585): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DMSLIGHPVK
Nearest Stachelhaus code(s):: Leu DAFLIGAAVK (50% 8� match); Ile DGLFIGIPVK (47% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (8265..8668): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DISDVAMVKK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (59% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_65 - Region 1 - indole

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Location: 198,601 - 229,341 nt. (total: 30,741 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_66 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 103,536 - 186,039 nt. (total: 82,504 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 103535 - 186039:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 103535 - 162398:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 109651 - 173316:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 117942 - 186039:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

580122

AMP-binding (41..354): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AYSFICQLTK
Nearest Stachelhaus code(s):: pNH2-Bz AYNFIAGSTK (26% 8� match)
Stachelhaus code match:: 60% (weak)

976739

AMP-binding (55..384): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGPSLGGLGK
Nearest Stachelhaus code(s):: Asn DGTKLGEVGK (35% 8� match)
Stachelhaus code match:: 60% (weak)

893951

PKS_AT (616..935): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; 2-Methylbutyryl-CoA: 62.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 78.9
Methylmalonyl-CoA: 78.7
Malonyl-CoA: 76.2
Ethylmalonyl-CoA: 57.2
Isobutyryl-CoA: 48.9
Propionyl-CoA: 43.5
2-Methylbutyryl-CoA: 37.4
inactive: 31.6
trans-1,2-CPDA: 29.4
fatty_acid: 26.1
Benzoyl-CoA: 18.5
Acetyl-CoA: 16.8
CHC-CoA: 16.5
3-Methylbutyryl-CoA: 15.5

scaffold_67 - Region 1 - fungal-RiPP-like

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Location: 130,093 - 221,233 nt. (total: 91,141 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_68 - Region 1 - fungal-RiPP-like

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Location: 70,893 - 168,486 nt. (total: 97,594 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_69 - Region 1 - T1PKS

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Location: 177,303 - 224,401 nt. (total: 47,099 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 177302 - 224401:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

976959

PKS_AT (536..853): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Isobutyryl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 83.2
Methoxymalonyl-CoA: 79.8
Isobutyryl-CoA: 67.8
Malonyl-CoA: 66.0
Ethylmalonyl-CoA: 42.8
Benzoyl-CoA: 28.9
Propionyl-CoA: 26.4
inactive: 24.8
2-Methylbutyryl-CoA: 24.7
3-Methylbutyryl-CoA: 23.2
trans-1,2-CPDA: 19.7
fatty_acid: 12.2
CHC-CoA: 10.8
Acetyl-CoA: 9.5

scaffold_7 - Region 1 - T1PKS

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Location: 211,693 - 279,606 nt. (total: 67,914 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 211692 - 279606:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

896751

PKS_AT (491..804): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 78.1
Malonyl-CoA: 76.9
Methoxymalonyl-CoA: 74.9
Isobutyryl-CoA: 54.6
Ethylmalonyl-CoA: 44.1
Propionyl-CoA: 40.0
fatty_acid: 23.2
trans-1,2-CPDA: 22.8
inactive: 20.4
Benzoyl-CoA: 20.2
3-Methylbutyryl-CoA: 18.0
2-Methylbutyryl-CoA: 17.2
CHC-CoA: 16.0
Acetyl-CoA: 6.8

scaffold_71 - Region 1 - T1PKS

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Location: 150,191 - 216,758 nt. (total: 66,568 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 150190 - 216758:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

894321

PKS_AT (908..1207): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 111.9
Methoxymalonyl-CoA: 67.4
inactive: 61.9
Methylmalonyl-CoA: 58.3
Propionyl-CoA: 33.6
Ethylmalonyl-CoA: 26.8
2-Methylbutyryl-CoA: 19.6
Isobutyryl-CoA: 15.4
Acetyl-CoA: 15.3
CHC-CoA: 14.6
3-Methylbutyryl-CoA: 12.0
Benzoyl-CoA: 11.3
fatty_acid: 10.9
trans-1,2-CPDA: 0.0

scaffold_75 - Region 1 - fungal-RiPP-like

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Location: 6,040 - 96,776 nt. (total: 90,737 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_76 - Region 1 - T1PKS

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Location: 1,580 - 69,599 nt. (total: 68,020 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 1579 - 69599:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

814222

PKS_AT (545..857): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 63.5
Methoxymalonyl-CoA: 60.7
Ethylmalonyl-CoA: 33.6
Propionyl-CoA: 27.3
Malonyl-CoA: 23.7
trans-1,2-CPDA: 22.2
Benzoyl-CoA: 15.7
Isobutyryl-CoA: 15.0
fatty_acid: 10.6
2-Methylbutyryl-CoA: 5.1
inactive: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_77 - Region 1 - NRPS,T1PKS

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Location: 1 - 72,010 nt. (total: 72,010 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 72010:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

894633

PKS_AT (554..874): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 79.5
Methoxymalonyl-CoA: 67.3
Ethylmalonyl-CoA: 60.7
Malonyl-CoA: 45.8
fatty_acid: 37.8
Propionyl-CoA: 29.2
trans-1,2-CPDA: 25.3
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 20.5
2-Methylbutyryl-CoA: 13.6
Acetyl-CoA: 9.5
CHC-CoA: 7.9
inactive: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (3129..3537): X

nrpys: MalAc

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: NHDKFGSISK
Nearest Stachelhaus code(s):: MalAc NHDKFGSISK (100% 8� match); Phe NHDKFGSISK (97% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_77 - Region 2 - NRPS-like,fungal-RiPP-like

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Location: 134,538 - 196,698 nt. (total: 62,161 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 134537 - 196698:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 136304 - 196698:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

939750

AMP-binding (80..417): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---LSATAIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_83 - Region 1 - fungal-RiPP-like

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Location: 1 - 59,669 nt. (total: 59,669 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_9 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 292,597 - 463,049 nt. (total: 170,453 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 292596 - 463049:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (pk)
Non-colinear ordering:: 800745 + 927847

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 292596 - 356175:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 337944 - 400334:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

800745

PKS_AT (573..890): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 80.2
Malonyl-CoA: 75.3
Methoxymalonyl-CoA: 74.1
Propionyl-CoA: 39.5
inactive: 39.2
2-Methylbutyryl-CoA: 36.7
Isobutyryl-CoA: 36.6
Ethylmalonyl-CoA: 28.4
fatty_acid: 25.8
trans-1,2-CPDA: 24.1
CHC-CoA: 20.9
Benzoyl-CoA: 19.8
Acetyl-CoA: 14.6
3-Methylbutyryl-CoA: 11.1

927847

PKS_AT (549..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 78.5
Malonyl-CoA: 78.1
Methoxymalonyl-CoA: 69.1
Isobutyryl-CoA: 48.7
Ethylmalonyl-CoA: 48.0
Benzoyl-CoA: 33.7
inactive: 33.6
Propionyl-CoA: 32.5
2-Methylbutyryl-CoA: 25.8
CHC-CoA: 15.6
trans-1,2-CPDA: 15.4
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_9 - Region 2 - T1PKS

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Location: 629,103 - 697,298 nt. (total: 68,196 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 7, location 629102 - 697298:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

818623

PKS_AT (527..845): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 85.5
Methoxymalonyl-CoA: 85.5
Malonyl-CoA: 77.9
Ethylmalonyl-CoA: 70.1
Propionyl-CoA: 48.9
Isobutyryl-CoA: 44.1
trans-1,2-CPDA: 42.2
2-Methylbutyryl-CoA: 32.4
Benzoyl-CoA: 17.2
fatty_acid: 16.4
inactive: 15.6
CHC-CoA: 12.6
3-Methylbutyryl-CoA: 11.8
Acetyl-CoA: 10.2

scaffold_90 - Region 1 - NRPS-like

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Location: 94,644 - 139,108 nt. (total: 44,465 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 94643 - 139108:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

876217

Search NORINE for peptide: strict or relaxed

AMP-binding (44..523): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (94% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_93 - Region 1 - NRPS-like,T1PKS

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Location: 43,383 - 120,270 nt. (total: 76,888 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 43382 - 120270:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 43382 - 111962:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 56936 - 120270:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

815560

PKS_AT (597..925): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 66.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 92.7
Methylmalonyl-CoA: 92.3
Malonyl-CoA: 75.8
Ethylmalonyl-CoA: 66.5
Isobutyryl-CoA: 62.3
2-Methylbutyryl-CoA: 45.3
Propionyl-CoA: 43.4
trans-1,2-CPDA: 37.6
fatty_acid: 29.8
Benzoyl-CoA: 27.9
inactive: 27.8
CHC-CoA: 26.7
Acetyl-CoA: 18.5
3-Methylbutyryl-CoA: 14.0

967874

AMP-binding (41..434): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AATLYGIVPK
Nearest Stachelhaus code(s):: Leu DAWLYGAVMK (38% 8� match); aIle DAFLYGGVFK (35% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_94 - Region 1 - indole

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Location: 68,379 - 100,343 nt. (total: 31,965 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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No matches found.

scaffold_95 - Region 1 - T1PKS

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Location: 1 - 79,207 nt. (total: 79,207 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 0 - 79207:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

901008

PKS_AT (514..808): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; 2-Methylbutyryl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 51.2
Methylmalonyl-CoA: 48.3
Isobutyryl-CoA: 31.6
Propionyl-CoA: 29.3
Ethylmalonyl-CoA: 21.3
inactive: 21.1
Malonyl-CoA: 15.2
3-Methylbutyryl-CoA: 14.7
trans-1,2-CPDA: 14.4
fatty_acid: 12.7
CHC-CoA: 8.6
2-Methylbutyryl-CoA: 3.3
Benzoyl-CoA: -0.6
Acetyl-CoA: -1.5

827160

PKS_AT (396..624): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 41.1
Methoxymalonyl-CoA: 40.1
Malonyl-CoA: 22.0
Ethylmalonyl-CoA: 20.2
fatty_acid: 15.2
2-Methylbutyryl-CoA: 13.5
CHC-CoA: 11.3
Propionyl-CoA: 9.6
Isobutyryl-CoA: 9.1
trans-1,2-CPDA: 0.0
inactive: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

895403

PKS_AT (581..887): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 71.1
Methoxymalonyl-CoA: 69.6
Malonyl-CoA: 57.6
Ethylmalonyl-CoA: 44.3
Isobutyryl-CoA: 34.3
Propionyl-CoA: 30.4
fatty_acid: 16.5
inactive: 15.1
CHC-CoA: 14.8
Benzoyl-CoA: 13.2
2-Methylbutyryl-CoA: 7.0
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_96 - Region 1 - T1PKS

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Location: 1 - 65,855 nt. (total: 65,855 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 0 - 65855:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

815647

PKS_AT (602..917): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 78.0
Methoxymalonyl-CoA: 75.1
Isobutyryl-CoA: 66.7
Malonyl-CoA: 63.5
Ethylmalonyl-CoA: 45.5
Propionyl-CoA: 32.3
2-Methylbutyryl-CoA: 28.8
trans-1,2-CPDA: 21.6
Benzoyl-CoA: 20.4
inactive: 17.8
3-Methylbutyryl-CoA: 13.1
fatty_acid: 9.5
CHC-CoA: 6.3
Acetyl-CoA: 0.0

scaffold_99 - Region 1 - terpene

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Location: 14,792 - 45,916 nt. (total: 31,125 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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