Piromyces finnis v3.0

Identified secondary metabolite regions using strictness 'relaxed'

scaffold_102

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS	9,697	59,532

scaffold_13

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	T1PKS	208,270	280,471

scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 112.1	T1PKS	674,957	746,287

scaffold_20

Region	Type	From	To	Most similar known cluster		Similarity
Region 113.1	T1PKS	656,169	722,842

scaffold_3

Region	Type	From	To	Most similar known cluster		Similarity
Region 156.1	T1PKS	460,572	527,279

scaffold_34

Region	Type	From	To	Most similar known cluster		Similarity
Region 161.1	T1PKS	150,723	217,921

scaffold_38

Region	Type	From	To	Most similar known cluster		Similarity
Region 165.1	T1PKS	232,885	302,332
Region 165.2	NRPS-like	334,552	399,586

scaffold_39

Region	Type	From	To	Most similar known cluster		Similarity
Region 166.1	T1PKS,NRPS-like	330,247	395,881

scaffold_5

Region	Type	From	To	Most similar known cluster		Similarity
Region 178.1	terpene	920,323	952,513

Region	Type	From	To	Similarity
Region 5.1	NRPS	9,697	59,532
Region 35.1	T1PKS	208,270	280,471
Region 112.1	T1PKS	674,957	746,287
Region 113.1	T1PKS	656,169	722,842
Region 156.1	T1PKS	460,572	527,279
Region 161.1	T1PKS	150,723	217,921
Region 165.1	T1PKS	232,885	302,332
Region 165.2	NRPS-like	334,552	399,586
Region 166.1	T1PKS,NRPS-like	330,247	395,881
Region 178.1	terpene	920,323	952,513

No secondary metabolite regions were found in these records:: scaffold_1; scaffold_10; scaffold_100; scaffold_101; scaffold_103; scaffold_104; scaffold_105; scaffold_106; scaffold_107; scaffold_108; scaffold_109; scaffold_11; scaffold_110; scaffold_111; scaffold_112; scaffold_113; scaffold_114; scaffold_115; scaffold_116; scaffold_117; scaffold_118; scaffold_119; scaffold_12; scaffold_120; scaffold_121; scaffold_122; scaffold_123; scaffold_124; scaffold_125; scaffold_126; scaffold_127; scaffold_128; scaffold_129; scaffold_130; scaffold_131; scaffold_132; scaffold_133; scaffold_134; scaffold_135; scaffold_136; scaffold_137; scaffold_138; scaffold_139; scaffold_14; scaffold_140; scaffold_141; scaffold_142; scaffold_143; scaffold_144; scaffold_145; scaffold_146; scaffold_147; scaffold_148; scaffold_149; scaffold_15; scaffold_150; scaffold_151; scaffold_152; scaffold_153; scaffold_154; scaffold_155; scaffold_156; scaffold_157; scaffold_158; scaffold_159; scaffold_16; scaffold_160; scaffold_161; scaffold_162; scaffold_163; scaffold_164; scaffold_165; scaffold_166; scaffold_167; scaffold_168; scaffold_169; scaffold_17; scaffold_170; scaffold_171; scaffold_172; scaffold_173; scaffold_174; scaffold_175; scaffold_176; scaffold_177; scaffold_178; scaffold_179; scaffold_18; scaffold_180; scaffold_181; scaffold_182; scaffold_183; scaffold_184; scaffold_185; scaffold_186; scaffold_187; scaffold_188; scaffold_189; scaffold_19; scaffold_190; scaffold_191; scaffold_192; scaffold_193; scaffold_194; scaffold_195; scaffold_196; scaffold_197; scaffold_198; scaffold_199; scaffold_200; scaffold_201; scaffold_202; scaffold_203; scaffold_204; scaffold_205; scaffold_206; scaffold_207; scaffold_208; scaffold_209; scaffold_21; scaffold_210; scaffold_211; scaffold_212; scaffold_213; scaffold_214; scaffold_215; scaffold_216; scaffold_217; scaffold_218; scaffold_219; scaffold_22; scaffold_220; scaffold_221; scaffold_222; scaffold_223; scaffold_224; scaffold_225; scaffold_226; scaffold_227; scaffold_228; scaffold_229; scaffold_23; scaffold_230; scaffold_231; scaffold_232; scaffold_24; scaffold_25; scaffold_26; scaffold_27; scaffold_28; scaffold_29; scaffold_30; scaffold_31; scaffold_32; scaffold_33; scaffold_35; scaffold_36; scaffold_37; scaffold_4; scaffold_40; scaffold_41; scaffold_42; scaffold_43; scaffold_44; scaffold_45; scaffold_46; scaffold_47; scaffold_48; scaffold_49; scaffold_50; scaffold_51; scaffold_52; scaffold_53; scaffold_54; scaffold_55; scaffold_56; scaffold_57; scaffold_58; scaffold_59; scaffold_6; scaffold_60; scaffold_61; scaffold_62; scaffold_63; scaffold_64; scaffold_65; scaffold_66; scaffold_67; scaffold_68; scaffold_69; scaffold_7; scaffold_70; scaffold_71; scaffold_72; scaffold_73; scaffold_74; scaffold_75; scaffold_76; scaffold_77; scaffold_78; scaffold_79; scaffold_8; scaffold_80; scaffold_81; scaffold_82; scaffold_83; scaffold_84; scaffold_85; scaffold_86; scaffold_87; scaffold_88; scaffold_89; scaffold_9; scaffold_90; scaffold_91; scaffold_92; scaffold_93; scaffold_94; scaffold_95; scaffold_96; scaffold_97; scaffold_98; scaffold_99

Compact view

scaffold_102 - Region 1 - NRPS

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Location: 9,697 - 59,532 nt. (total: 49,836 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

416967

AMP-binding (50..460): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IMAVGGGSMK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (818..1197): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIGFLGVVVK
Nearest Stachelhaus code(s):: Ile DGFFLGVVYK (50% 8� match); Gly DILQLGVVWK (47% 8� match); Leu DAWFLGNVVK (38% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_13 - Region 1 - T1PKS

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Location: 208,270 - 280,471 nt. (total: 72,202 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 208269 - 280471:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

411080

PKS_AT (1015..1310): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; Benzoyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 78.3
Methoxymalonyl-CoA: 66.4
Malonyl-CoA: 60.7
Benzoyl-CoA: 39.6
Propionyl-CoA: 34.8
Isobutyryl-CoA: 34.8
Ethylmalonyl-CoA: 34.1
fatty_acid: 33.5
inactive: 29.1
2-Methylbutyryl-CoA: 27.5
CHC-CoA: 24.2
trans-1,2-CPDA: 14.7
3-Methylbutyryl-CoA: 8.2
Acetyl-CoA: 0.0

scaffold_2 - Region 1 - T1PKS

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Location: 674,957 - 746,287 nt. (total: 71,331 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

316229

PKS_AT (958..1241): pk

ATSignature: (unknown)

No matches above 50%

minowa: Benzoyl-CoA

Prediction, score:

Benzoyl-CoA: 15.6
Methylmalonyl-CoA: 14.3
Methoxymalonyl-CoA: 12.2
Propionyl-CoA: 10.1
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

408350

AMP-binding (40..484): X

scaffold_20 - Region 1 - T1PKS

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Location: 656,169 - 722,842 nt. (total: 66,674 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

412264

PKS_AT (932..1222): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 42.2
Methoxymalonyl-CoA: 42.0
Malonyl-CoA: 40.1
Benzoyl-CoA: 33.8
inactive: 32.4
Isobutyryl-CoA: 23.4
2-Methylbutyryl-CoA: 13.7
3-Methylbutyryl-CoA: 12.2
CHC-CoA: 11.7
Propionyl-CoA: 10.9
fatty_acid: 10.0
Ethylmalonyl-CoA: 8.2
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0

scaffold_3 - Region 1 - T1PKS

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Location: 460,572 - 527,279 nt. (total: 66,708 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 460571 - 527279:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

342883

PKS_AT (634..929): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 92.5
inactive: 85.4
Methoxymalonyl-CoA: 53.2
Methylmalonyl-CoA: 34.8
Propionyl-CoA: 31.7
2-Methylbutyryl-CoA: 18.0
Benzoyl-CoA: 15.1
trans-1,2-CPDA: 14.4
Isobutyryl-CoA: 12.6
CHC-CoA: 12.4
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_34 - Region 1 - T1PKS

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Location: 150,723 - 217,921 nt. (total: 67,199 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 150722 - 217921:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

413919

PKS_AT (638..940): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 86.8
inactive: 76.4
Methoxymalonyl-CoA: 60.0
Methylmalonyl-CoA: 46.0
Propionyl-CoA: 27.8
2-Methylbutyryl-CoA: 21.4
Benzoyl-CoA: 18.2
Isobutyryl-CoA: 15.7
CHC-CoA: 15.1
Ethylmalonyl-CoA: 11.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_38 - Region 1 - T1PKS

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Location: 232,885 - 302,332 nt. (total: 69,448 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

414372

PKS_AT (1008..1298): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 34.4
Methylmalonyl-CoA: 32.7
trans-1,2-CPDA: 13.9
3-Methylbutyryl-CoA: 10.2
Propionyl-CoA: 10.1
Benzoyl-CoA: 9.5
Isobutyryl-CoA: 0.4
inactive: 0.0
fatty_acid: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_38 - Region 2 - NRPS-like

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Location: 334,552 - 399,586 nt. (total: 65,035 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 334551 - 399586:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

357934

Search NORINE for peptide: strict or relaxed

AMP-binding (264..716): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (94% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_39 - Region 1 - NRPS-like,T1PKS

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Location: 330,247 - 395,881 nt. (total: 65,635 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 330246 - 395881:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

358210

AMP-binding (70..513): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LLQLQGSVMK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_5 - Region 1 - terpene

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Location: 920,323 - 952,513 nt. (total: 32,191 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.