Fitzroyomyces cyperi CBS 143170 v1.0

Identified secondary metabolite regions using strictness 'relaxed'

scaffold_1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	fungal-RiPP-like	726,103	813,588
Region 1.2	NRPS-like	873,805	931,810
Region 1.3	fungal-RiPP-like	1,087,349	1,175,354
Region 1.4	terpene	1,636,540	1,667,646
Region 1.5	NRPS-like	2,500,656	2,561,415
Region 1.6	fungal-RiPP-like	2,780,599	2,891,407
Region 1.7	indole	3,151,121	3,182,520
Region 1.8	NRPS	3,213,536	3,279,523	metachelin C/metachelin A/metachelin A-CE/metachelin B/dimerumic acid 11-mannoside/dimerumic acid	NRP	37%

scaffold_10

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	818,961	850,560	squalestatin S1	Terpene	40%
Region 2.2	fungal-RiPP-like	1,854,812	1,945,075

scaffold_11

Region	Type	From	To
Region 3.1	fungal-RiPP-like	1	65,645
Region 3.2	NRPS-like,T1PKS	115,309	200,344
Region 3.3	terpene	592,346	624,612
Region 3.4	T1PKS	1,841,953	1,908,983

scaffold_12

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	terpene,fungal-RiPP-like	185,259	276,842
Region 4.2	T1PKS	779,991	844,053	cichorine	Polyketide	100%
Region 4.3	T1PKS	1,105,204	1,170,662	mycophenolic acid	Polyketide	25%
Region 4.4	T1PKS	1,285,063	1,350,552
Region 4.5	terpene	1,603,783	1,635,050
Region 4.6	T1PKS	1,848,165	1,897,549

scaffold_13

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	T1PKS	8,104	79,888	tricholignan A	Polyketide:Iterative type I polyketide	22%
Region 5.2	T3PKS	1,656,390	1,717,646
Region 5.3	T1PKS	1,826,590	1,890,979

scaffold_14

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS-like	733,529	796,794
Region 6.2	T1PKS	1,734,718	1,803,486

scaffold_15

Region	Type	From	To
Region 7.1	fungal-RiPP-like	160,321	249,841
Region 7.2	indole	453,362	486,215
Region 7.3	T1PKS,fungal-RiPP-like	590,349	674,060
Region 7.4	fungal-RiPP-like	1,129,488	1,238,526

scaffold_16

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	247,440	312,677	aspirochlorine	NRP	22%
Region 8.2	fungal-RiPP-like	461,285	552,202

scaffold_2

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	fungal-RiPP-like	642,822	730,013	citrinin	Polyketide:Iterative type I polyketide	12%
Region 11.2	fungal-RiPP-like	928,005	1,018,942
Region 11.3	NRPS	1,124,542	1,208,121
Region 11.4	T1PKS	2,394,548	2,461,223
Region 11.5	T1PKS	2,806,006	2,874,191	solanapyrone A	Polyketide	40%

scaffold_3

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	fungal-RiPP-like	367,548	456,091
Region 12.2	fungal-RiPP-like	563,499	652,467
Region 12.3	indole	1,915,387	1,946,774	biscognienyne B/eutypinic acid/compound 3/4-hydroxy-3-prenylbenzoic acid/prenylhydroquinone/compound 15/compound 8/compound 7/compound 6/compound 11/compound 12/pestalodiol E	Other	54%
Region 12.4	NRPS	2,090,715	2,148,312	penigainamide A/penigainamide B/penigainamide C/adametizine A/FA2097/outovirin A/outovirin C/pretrichodermamide C	Alkaloid	61%
Region 12.5	T1PKS	2,300,540	2,366,676
Region 12.6	fungal-RiPP-like,NRPS	2,491,866	2,591,030	cyclochlorotine/hydroxycyclochlorotine A/cyclotine/cyclochlorotine B/deoxy-Cyclochlorotine	NRP	66%

scaffold_4

Region	Type	From	To
Region 13.1	terpene	95,373	122,410
Region 13.2	fungal-RiPP-like	932,319	1,019,698
Region 13.3	NRPS-like,fungal-RiPP-like	1,677,987	1,798,983
Region 13.4	fungal-RiPP-like	2,093,531	2,241,968
Region 13.5	NRPS-like,terpene	2,288,477	2,379,612
Region 13.6	fungal-RiPP-like	2,406,274	2,497,997

scaffold_5

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	fungal-RiPP-like,NRPS	265	101,181	cyclochlorotine/hydroxycyclochlorotine A/cyclotine/cyclochlorotine B/deoxy-Cyclochlorotine	NRP	83%
Region 14.2	T1PKS	519,884	582,692	patulin	Polyketide:Iterative type I polyketide	53%
Region 14.3	fungal-RiPP-like	689,205	780,189
Region 14.4	T3PKS	795,612	857,262
Region 14.5	T1PKS	2,296,090	2,363,523

scaffold_6

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	T1PKS	229,778	292,475
Region 15.2	terpene	1,026,566	1,057,658
Region 15.3	T1PKS	1,393,339	1,463,126	viriditoxin	Polyketide	33%
Region 15.4	NRPS-like	2,073,771	2,137,015

scaffold_7

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	T1PKS	242,762	305,690	4-oxomacrophorin A/macrophorin A/5'-epimacrophorin B	Terpene	72%
Region 16.2	indole	441,744	473,054
Region 16.3	T1PKS	1,414,029	1,479,632
Region 16.4	T1PKS	1,845,523	1,912,928	depudecin	Polyketide:Iterative type I polyketide	50%
Region 16.5	fungal-RiPP-like	1,987,936	2,072,994
Region 16.6	fungal-RiPP-like	2,158,589	2,249,452
Region 16.7	terpene	2,264,802	2,296,281

scaffold_8

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	fungal-RiPP-like	6,971	67,261
Region 17.2	NRPS-like,fungal-RiPP-like	294,824	433,907
Region 17.3	NRPS-like	847,780	906,327
Region 17.4	NRPS,T1PKS	913,712	985,085
Region 17.5	T1PKS	1,109,952	1,175,396	sordarial	Polyketide	40%
Region 17.6	fungal-RiPP-like,T1PKS	1,178,172	1,354,467
Region 17.7	NRPS-like	2,039,746	2,102,643

scaffold_9

Region	Type	From	To
Region 18.1	NRP-metallophore,NRPS,fungal-RiPP-like	73	105,598
Region 18.2	fungal-RiPP-like	1,182,337	1,273,504
Region 18.3	NRPS-like	1,404,781	1,460,638
Region 18.4	NRPS-like	1,933,095	1,990,915

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	fungal-RiPP-like	726,103	813,588
Region 1.2	NRPS-like	873,805	931,810
Region 1.3	fungal-RiPP-like	1,087,349	1,175,354
Region 1.4	terpene	1,636,540	1,667,646
Region 1.5	NRPS-like	2,500,656	2,561,415
Region 1.6	fungal-RiPP-like	2,780,599	2,891,407
Region 1.7	indole	3,151,121	3,182,520
Region 1.8	NRPS	3,213,536	3,279,523	metachelin C/metachelin A/metachelin A-CE/metachelin B/dimerumic acid 11-mannoside/dimerumic acid	NRP	37%
Region 2.1	terpene	818,961	850,560	squalestatin S1	Terpene	40%
Region 2.2	fungal-RiPP-like	1,854,812	1,945,075
Region 3.1	fungal-RiPP-like	1	65,645
Region 3.2	NRPS-like,T1PKS	115,309	200,344
Region 3.3	terpene	592,346	624,612
Region 3.4	T1PKS	1,841,953	1,908,983
Region 4.1	terpene,fungal-RiPP-like	185,259	276,842
Region 4.2	T1PKS	779,991	844,053	cichorine	Polyketide	100%
Region 4.3	T1PKS	1,105,204	1,170,662	mycophenolic acid	Polyketide	25%
Region 4.4	T1PKS	1,285,063	1,350,552
Region 4.5	terpene	1,603,783	1,635,050
Region 4.6	T1PKS	1,848,165	1,897,549
Region 5.1	T1PKS	8,104	79,888	tricholignan A	Polyketide:Iterative type I polyketide	22%
Region 5.2	T3PKS	1,656,390	1,717,646
Region 5.3	T1PKS	1,826,590	1,890,979
Region 6.1	NRPS-like	733,529	796,794
Region 6.2	T1PKS	1,734,718	1,803,486
Region 7.1	fungal-RiPP-like	160,321	249,841
Region 7.2	indole	453,362	486,215
Region 7.3	T1PKS,fungal-RiPP-like	590,349	674,060
Region 7.4	fungal-RiPP-like	1,129,488	1,238,526
Region 8.1	NRPS	247,440	312,677	aspirochlorine	NRP	22%
Region 8.2	fungal-RiPP-like	461,285	552,202
Region 11.1	fungal-RiPP-like	642,822	730,013	citrinin	Polyketide:Iterative type I polyketide	12%
Region 11.2	fungal-RiPP-like	928,005	1,018,942
Region 11.3	NRPS	1,124,542	1,208,121
Region 11.4	T1PKS	2,394,548	2,461,223
Region 11.5	T1PKS	2,806,006	2,874,191	solanapyrone A	Polyketide	40%
Region 12.1	fungal-RiPP-like	367,548	456,091
Region 12.2	fungal-RiPP-like	563,499	652,467
Region 12.3	indole	1,915,387	1,946,774	biscognienyne B/eutypinic acid/compound 3/4-hydroxy-3-prenylbenzoic acid/prenylhydroquinone/compound 15/compound 8/compound 7/compound 6/compound 11/compound 12/pestalodiol E	Other	54%
Region 12.4	NRPS	2,090,715	2,148,312	penigainamide A/penigainamide B/penigainamide C/adametizine A/FA2097/outovirin A/outovirin C/pretrichodermamide C	Alkaloid	61%
Region 12.5	T1PKS	2,300,540	2,366,676
Region 12.6	fungal-RiPP-like,NRPS	2,491,866	2,591,030	cyclochlorotine/hydroxycyclochlorotine A/cyclotine/cyclochlorotine B/deoxy-Cyclochlorotine	NRP	66%
Region 13.1	terpene	95,373	122,410
Region 13.2	fungal-RiPP-like	932,319	1,019,698
Region 13.3	NRPS-like,fungal-RiPP-like	1,677,987	1,798,983
Region 13.4	fungal-RiPP-like	2,093,531	2,241,968
Region 13.5	NRPS-like,terpene	2,288,477	2,379,612
Region 13.6	fungal-RiPP-like	2,406,274	2,497,997
Region 14.1	fungal-RiPP-like,NRPS	265	101,181	cyclochlorotine/hydroxycyclochlorotine A/cyclotine/cyclochlorotine B/deoxy-Cyclochlorotine	NRP	83%
Region 14.2	T1PKS	519,884	582,692	patulin	Polyketide:Iterative type I polyketide	53%
Region 14.3	fungal-RiPP-like	689,205	780,189
Region 14.4	T3PKS	795,612	857,262
Region 14.5	T1PKS	2,296,090	2,363,523
Region 15.1	T1PKS	229,778	292,475
Region 15.2	terpene	1,026,566	1,057,658
Region 15.3	T1PKS	1,393,339	1,463,126	viriditoxin	Polyketide	33%
Region 15.4	NRPS-like	2,073,771	2,137,015
Region 16.1	T1PKS	242,762	305,690	4-oxomacrophorin A/macrophorin A/5'-epimacrophorin B	Terpene	72%
Region 16.2	indole	441,744	473,054
Region 16.3	T1PKS	1,414,029	1,479,632
Region 16.4	T1PKS	1,845,523	1,912,928	depudecin	Polyketide:Iterative type I polyketide	50%
Region 16.5	fungal-RiPP-like	1,987,936	2,072,994
Region 16.6	fungal-RiPP-like	2,158,589	2,249,452
Region 16.7	terpene	2,264,802	2,296,281
Region 17.1	fungal-RiPP-like	6,971	67,261
Region 17.2	NRPS-like,fungal-RiPP-like	294,824	433,907
Region 17.3	NRPS-like	847,780	906,327
Region 17.4	NRPS,T1PKS	913,712	985,085
Region 17.5	T1PKS	1,109,952	1,175,396	sordarial	Polyketide	40%
Region 17.6	fungal-RiPP-like,T1PKS	1,178,172	1,354,467
Region 17.7	NRPS-like	2,039,746	2,102,643
Region 18.1	NRP-metallophore,NRPS,fungal-RiPP-like	73	105,598
Region 18.2	fungal-RiPP-like	1,182,337	1,273,504
Region 18.3	NRPS-like	1,404,781	1,460,638
Region 18.4	NRPS-like	1,933,095	1,990,915

No secondary metabolite regions were found in these records:: scaffold_17; scaffold_18

Compact view

scaffold_1 - Region 1 - fungal-RiPP-like

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Location: 726,103 - 813,588 nt. (total: 87,486 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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scaffold_1 - Region 2 - NRPS-like

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Location: 873,805 - 931,810 nt. (total: 58,006 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 873804 - 931810:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

448909

Search NORINE for peptide: strict or relaxed

AMP-binding (23..489): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (100% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_1 - Region 3 - fungal-RiPP-like

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Location: 1,087,349 - 1,175,354 nt. (total: 88,006 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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core biosynthetic genes

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scaffold_1 - Region 4 - terpene

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Location: 1,636,540 - 1,667,646 nt. (total: 31,107 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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		NT	AA		NT	AA

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scaffold_1 - Region 5 - NRPS-like

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Location: 2,500,656 - 2,561,415 nt. (total: 60,760 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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resistance

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 2500655 - 2561415:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

23033

AMP-binding (55..432): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --FYIAISVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_1 - Region 6 - fungal-RiPP-like

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Location: 2,780,599 - 2,891,407 nt. (total: 110,809 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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core biosynthetic genes

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		NT	AA		NT	AA

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scaffold_1 - Region 7 - indole

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Location: 3,151,121 - 3,182,520 nt. (total: 31,400 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_1 - Region 8 - NRPS

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Location: 3,213,536 - 3,279,523 nt. (total: 65,988 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 8, location 3213535 - 3279523:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

484571

AMP-binding (221..628): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVDHGGAVGK
Nearest Stachelhaus code(s):: Z-ahmohOrn DVDGGGGIGK (68% 8� match)
Stachelhaus code match:: 70% (weak)

512590

AMP-binding (54..487): X

nrpys: Z-ahmohOrn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGLHIASTCK
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (94% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_10 - Region 1 - terpene

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Location: 818,961 - 850,560 nt. (total: 31,600 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

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transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

scaffold_10 - Region 2 - fungal-RiPP-like

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Location: 1,854,812 - 1,945,075 nt. (total: 90,264 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_11 - Region 1 - fungal-RiPP-like

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Location: 1 - 65,645 nt. (total: 65,645 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_11 - Region 2 - NRPS-like,T1PKS

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Location: 115,309 - 200,344 nt. (total: 85,036 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 115308 - 200344:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 115308 - 178638:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 137226 - 200344:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

473362

AMP-binding (23..356): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --YFMATLHK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

405837

PKS_AT (609..933): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.8
Methoxymalonyl-CoA: 71.1
Malonyl-CoA: 47.7
Ethylmalonyl-CoA: 47.0
Isobutyryl-CoA: 34.4
trans-1,2-CPDA: 27.8
Propionyl-CoA: 25.6
Benzoyl-CoA: 22.6
2-Methylbutyryl-CoA: 22.4
fatty_acid: 19.4
inactive: 10.6
3-Methylbutyryl-CoA: 9.7
CHC-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_11 - Region 3 - terpene

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Location: 592,346 - 624,612 nt. (total: 32,267 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_11 - Region 4 - T1PKS

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Location: 1,841,953 - 1,908,983 nt. (total: 67,031 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 6, location 1841952 - 1908983:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

64517

PKS_AT (579..893): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 78.7
Methoxymalonyl-CoA: 77.2
Ethylmalonyl-CoA: 53.1
Malonyl-CoA: 51.4
Isobutyryl-CoA: 50.9
Propionyl-CoA: 28.8
2-Methylbutyryl-CoA: 25.2
inactive: 24.7
Benzoyl-CoA: 23.0
trans-1,2-CPDA: 21.8
fatty_acid: 14.2
CHC-CoA: 12.9
Acetyl-CoA: 11.3
3-Methylbutyryl-CoA: 8.0

scaffold_12 - Region 1 - fungal-RiPP-like,terpene

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Location: 185,259 - 276,842 nt. (total: 91,584 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)
fungal-RiPP-like: DUF3328

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scaffold_12 - Region 2 - T1PKS

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Location: 779,991 - 844,053 nt. (total: 64,063 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 4, location 779990 - 844053:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

72557

PKS_AT (922..1218): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: inactive

Prediction, score:

inactive: 59.7
Malonyl-CoA: 59.7
Methoxymalonyl-CoA: 48.6
Methylmalonyl-CoA: 38.8
Ethylmalonyl-CoA: 25.4
Benzoyl-CoA: 22.2
Propionyl-CoA: 21.0
fatty_acid: 19.8
CHC-CoA: 18.8
2-Methylbutyryl-CoA: 16.2
Isobutyryl-CoA: 14.0
trans-1,2-CPDA: 12.2
Acetyl-CoA: 9.1
3-Methylbutyryl-CoA: 8.9

scaffold_12 - Region 3 - T1PKS

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Location: 1,105,204 - 1,170,662 nt. (total: 65,459 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 5, location 1105203 - 1170662:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

75797

PKS_AT (908..1201): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 52.6
Methoxymalonyl-CoA: 43.6
inactive: 41.8
Methylmalonyl-CoA: 37.6
Ethylmalonyl-CoA: 19.3
Propionyl-CoA: 16.4
fatty_acid: 13.3
2-Methylbutyryl-CoA: 13.0
Benzoyl-CoA: 12.5
CHC-CoA: 12.2
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_12 - Region 4 - T1PKS

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Location: 1,285,063 - 1,350,552 nt. (total: 65,490 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 6, location 1285062 - 1350552:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

427827

PKS_AT (548..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; 2-Methylbutyryl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 47.7
Methoxymalonyl-CoA: 35.8
Malonyl-CoA: 18.6
Propionyl-CoA: 16.5
CHC-CoA: 15.5
Benzoyl-CoA: 13.3
2-Methylbutyryl-CoA: 12.3
Ethylmalonyl-CoA: 12.2
fatty_acid: 11.3
Isobutyryl-CoA: 6.5
trans-1,2-CPDA: 0.0
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_12 - Region 5 - terpene

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Location: 1,603,783 - 1,635,050 nt. (total: 31,268 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_12 - Region 6 - T1PKS

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Location: 1,848,165 - 1,897,549 nt. (total: 49,385 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 8, location 1848164 - 1897549:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

387503

PKS_AT (925..1223): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 52.6
Methoxymalonyl-CoA: 37.5
Methylmalonyl-CoA: 23.4
inactive: 17.4
Isobutyryl-CoA: 13.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_13 - Region 1 - T1PKS

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Location: 8,104 - 79,888 nt. (total: 71,785 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 8103 - 79888:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 407960 + 82296

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

407960

PKS_AT (556..872): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 91.0
Methoxymalonyl-CoA: 76.8
Ethylmalonyl-CoA: 61.8
Malonyl-CoA: 53.1
Isobutyryl-CoA: 51.7
2-Methylbutyryl-CoA: 42.2
Propionyl-CoA: 38.6
Benzoyl-CoA: 29.4
CHC-CoA: 26.0
trans-1,2-CPDA: 25.7
inactive: 18.4
3-Methylbutyryl-CoA: 14.5
fatty_acid: 11.9
Acetyl-CoA: 9.7

82296

PKS_AT (898..1203): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 62.5%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.4
inactive: 58.0
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 47.7
Isobutyryl-CoA: 26.6
Benzoyl-CoA: 21.0
Propionyl-CoA: 17.1
fatty_acid: 14.4
CHC-CoA: 13.6
Ethylmalonyl-CoA: 10.9
Acetyl-CoA: 10.4
2-Methylbutyryl-CoA: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_13 - Region 2 - T3PKS

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Location: 1,656,390 - 1,717,646 nt. (total: 61,257 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_13 - Region 3 - T1PKS

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Location: 1,826,590 - 1,890,979 nt. (total: 64,390 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 3, location 1826589 - 1890979:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

478811

PKS_AT (639..947): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 55.8
Methylmalonyl-CoA: 55.3
Methoxymalonyl-CoA: 51.2
Ethylmalonyl-CoA: 23.4
Propionyl-CoA: 17.7
3-Methylbutyryl-CoA: 14.0
fatty_acid: 13.1
Benzoyl-CoA: 11.1
inactive: 9.5
Isobutyryl-CoA: 8.3
CHC-CoA: 6.7
2-Methylbutyryl-CoA: 4.6
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0

scaffold_14 - Region 1 - NRPS-like

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Location: 733,529 - 796,794 nt. (total: 63,266 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 733528 - 796794:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

503999

AMP-binding (60..352): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -HAHLGHLMK
Nearest Stachelhaus code(s):: Hpg DAAHLGLLCK (32% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_14 - Region 2 - T1PKS

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Location: 1,734,718 - 1,803,486 nt. (total: 68,769 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 1734717 - 1803486:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

111374

PKS_AT (551..868): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 74.0
Ethylmalonyl-CoA: 64.8
Methoxymalonyl-CoA: 60.0
Isobutyryl-CoA: 44.2
Malonyl-CoA: 38.9
Benzoyl-CoA: 38.4
inactive: 18.9
fatty_acid: 17.3
Acetyl-CoA: 15.9
trans-1,2-CPDA: 13.0
Propionyl-CoA: 12.4
CHC-CoA: 8.1
2-Methylbutyryl-CoA: 7.0
3-Methylbutyryl-CoA: 0.0

389175

AMP-binding (27..432): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AGNHWGVWIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_15 - Region 1 - fungal-RiPP-like

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Location: 160,321 - 249,841 nt. (total: 89,521 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_15 - Region 2 - indole

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Location: 453,362 - 486,215 nt. (total: 32,854 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_15 - Region 3 - T1PKS,fungal-RiPP-like

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Location: 590,349 - 674,060 nt. (total: 83,712 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 590348 - 674060:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 590348 - 658645:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

116981

PKS_AT (578..908): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 72.0
Methoxymalonyl-CoA: 57.0
Malonyl-CoA: 47.9
Ethylmalonyl-CoA: 43.2
Isobutyryl-CoA: 37.5
Propionyl-CoA: 33.3
trans-1,2-CPDA: 24.7
inactive: 21.2
fatty_acid: 21.2
Benzoyl-CoA: 17.7
2-Methylbutyryl-CoA: 15.8
CHC-CoA: 12.3
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_15 - Region 4 - fungal-RiPP-like

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Location: 1,129,488 - 1,238,526 nt. (total: 109,039 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_16 - Region 1 - NRPS

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Location: 247,440 - 312,677 nt. (total: 65,238 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 247439 - 312677:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

510584

Search NORINE for peptide: strict or relaxed

AMP-binding (570..952): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGHIYVMCGK
Nearest Stachelhaus code(s):: Phe DGHIYVMCGK (91% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_16 - Region 2 - fungal-RiPP-like

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Location: 461,285 - 552,202 nt. (total: 90,918 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_2 - Region 1 - fungal-RiPP-like

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Location: 642,822 - 730,013 nt. (total: 87,192 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_2 - Region 2 - fungal-RiPP-like

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Location: 928,005 - 1,018,942 nt. (total: 90,938 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_2 - Region 3 - NRPS

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Location: 1,124,542 - 1,208,121 nt. (total: 83,580 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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No matches found.

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1124541 - 1208121:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X - D-Leu - Leu - D-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

144556

Search NORINE for peptide: strict or relaxed

AMP-binding (245..638): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVTLVGCVVK
Nearest Stachelhaus code(s):: Trp DVHEVGAVVK (62% 8� match); Pro DVMLVGAVNK (59% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1789..2192): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNADAGCINK
Nearest Stachelhaus code(s):: Lys DVAHPGFINK (47% 8� match); N6-ohLys DAQDAGCVEK (44% 8� match); Arg DVADVGAIDK (38% 8� match)
Stachelhaus code match:: 60% (weak)

AMP-binding (2873..3270): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMLVGAVVK
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (56% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4414..4819): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMLVGAVIK
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (56% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (5509..5909): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIGFAGGVFK
Nearest Stachelhaus code(s):: Val DGFFAGGVFK (53% 8� match)
Stachelhaus code match:: 80% (moderate)

533852

AMP-binding (131..523): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ALTV---LAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_2 - Region 4 - T1PKS

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Location: 2,394,548 - 2,461,223 nt. (total: 66,676 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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No matches found.

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 4, location 2394547 - 2461223:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

157489

PKS_AT (560..874): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Ethylmalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 59.1
Methoxymalonyl-CoA: 54.4
Malonyl-CoA: 46.2
Isobutyryl-CoA: 26.8
Propionyl-CoA: 24.6
Ethylmalonyl-CoA: 23.3
3-Methylbutyryl-CoA: 11.6
Benzoyl-CoA: 10.1
CHC-CoA: 6.9
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaffold_2 - Region 5 - T1PKS

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Location: 2,806,006 - 2,874,191 nt. (total: 68,186 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 2806005 - 2874191:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

534123

PKS_AT (596..911): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Ethylmalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 58.1
Methylmalonyl-CoA: 57.6
Malonyl-CoA: 35.0
Ethylmalonyl-CoA: 31.4
Propionyl-CoA: 29.5
CHC-CoA: 24.1
Isobutyryl-CoA: 20.8
fatty_acid: 17.2
2-Methylbutyryl-CoA: 14.5
inactive: 12.6
3-Methylbutyryl-CoA: 12.6
trans-1,2-CPDA: 12.1
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_3 - Region 1 - fungal-RiPP-like

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Location: 367,548 - 456,091 nt. (total: 88,544 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_3 - Region 2 - fungal-RiPP-like

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Location: 563,499 - 652,467 nt. (total: 88,969 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_3 - Region 3 - indole

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Location: 1,915,387 - 1,946,774 nt. (total: 31,388 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_3 - Region 4 - NRPS

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Location: 2,090,715 - 2,148,312 nt. (total: 57,598 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 4, location 2090714 - 2148312:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

506509

Search NORINE for peptide: strict or relaxed

AMP-binding (433..800): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGHLYVMGGK
Nearest Stachelhaus code(s):: Phe DGHIYVMCGK (85% 8� match)
Stachelhaus code match:: 80% (moderate)

scaffold_3 - Region 5 - T1PKS

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Location: 2,300,540 - 2,366,676 nt. (total: 66,137 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 2300539 - 2366676:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

375259

PKS_AT (891..1193): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.8
inactive: 52.7
Methylmalonyl-CoA: 34.0
Methoxymalonyl-CoA: 31.0
Isobutyryl-CoA: 23.5
2-Methylbutyryl-CoA: 22.5
Benzoyl-CoA: 21.0
Ethylmalonyl-CoA: 16.6
Propionyl-CoA: 14.2
CHC-CoA: 14.0
3-Methylbutyryl-CoA: 10.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0

scaffold_3 - Region 6 - NRPS,fungal-RiPP-like

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Location: 2,491,866 - 2,591,030 nt. (total: 99,165 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 6, location 2491865 - 2591030:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Pro - Val - Ser - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 2521596 - 2591030:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Pro - Val - Ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

457111

Search NORINE for peptide: strict or relaxed

AMP-binding (213..618): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGQECSVTK
Nearest Stachelhaus code(s):: Ser DVGQESTITK (62% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1368..1760): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIHDVSAICK
Nearest Stachelhaus code(s):: Pro DIHDVSAICK (88% 8� match); clPro or Pro DIHDVSAICK (85% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2448..2849): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DLWFFGLAIK
Nearest Stachelhaus code(s):: Ser DVWFFGLVDK (56% 8� match); Leu or Arg DVWFFGLVDK (50% 8� match)
Stachelhaus code match:: 70% (weak)

AMP-binding (3507..3905): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVNEISCSVK
Nearest Stachelhaus code(s):: Ser DVNEISCSVK (97% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4604..5009): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFQVTLILK
Nearest Stachelhaus code(s):: bPhe DAFQVTVIMK (82% 8� match)
Stachelhaus code match:: 80% (moderate)

scaffold_4 - Region 1 - terpene

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Location: 95,373 - 122,410 nt. (total: 27,038 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_4 - Region 2 - fungal-RiPP-like

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Location: 932,319 - 1,019,698 nt. (total: 87,380 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_4 - Region 3 - NRPS-like,fungal-RiPP-like

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Location: 1,677,987 - 1,798,983 nt. (total: 120,997 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
fungal-RiPP-like: DUF3328

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Predicted core structure(s)

For candidate cluster 3, location 1677986 - 1798983:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 1677986 - 1728962:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

458822

AMP-binding (19..337): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AAATIGWIVK
Nearest Stachelhaus code(s):: Trp DAACIGNVVK (32% 8� match); Leu DAWIIGAIVK (29% 8� match)
Stachelhaus code match:: 60% (weak)

541783

AMP-binding (16..320): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GGTMCA---K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_4 - Region 4 - fungal-RiPP-like

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Location: 2,093,531 - 2,241,968 nt. (total: 148,438 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_4 - Region 5 - NRPS-like,terpene

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Location: 2,288,477 - 2,379,612 nt. (total: 91,136 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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No matches found.

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 9, location 2288476 - 2379612:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 10, location 2288476 - 2350794:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

204154

AMP-binding (37..357): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AAGVSVLWYK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_4 - Region 6 - fungal-RiPP-like

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Location: 2,406,274 - 2,497,997 nt. (total: 91,724 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_5 - Region 1 - NRPS,fungal-RiPP-like

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Location: 265 - 101,181 nt. (total: 100,917 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 264 - 101181:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Pro - Abu - Ser - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 7848 - 84903:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Pro - Abu - Ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

440805

Search NORINE for peptide: strict or relaxed

AMP-binding (208..610): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMGQESTVTK
Nearest Stachelhaus code(s):: Ser DVGQESTITK (65% 8� match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1376..1766): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIHDVSGICK
Nearest Stachelhaus code(s):: clPro or Pro DIHDVSAICK (88% 8� match); Pro DIHDVSAICK (79% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2451..2853): Abu

nrpys: Abu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFYGAPLK
Nearest Stachelhaus code(s):: Abu DAWFYGAPLK (94% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3511..3916): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVNEISCSVK
Nearest Stachelhaus code(s):: Ser DVNEISCSVK (88% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4617..5021): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DACQVTAVLK
Nearest Stachelhaus code(s):: Leu DASLVGAVLK (47% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_5 - Region 2 - T1PKS

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Location: 519,884 - 582,692 nt. (total: 62,809 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 519883 - 582692:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

440965

PKS_AT (570..861): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 108.5
Methoxymalonyl-CoA: 92.8
Ethylmalonyl-CoA: 82.4
Malonyl-CoA: 68.0
Isobutyryl-CoA: 64.7
Propionyl-CoA: 50.9
2-Methylbutyryl-CoA: 49.3
3-Methylbutyryl-CoA: 46.9
trans-1,2-CPDA: 46.4
Acetyl-CoA: 34.7
Benzoyl-CoA: 28.3
fatty_acid: 27.5
CHC-CoA: 17.2
inactive: 8.0

scaffold_5 - Region 3 - fungal-RiPP-like

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Location: 689,205 - 780,189 nt. (total: 90,985 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_5 - Region 4 - T3PKS

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Location: 795,612 - 857,262 nt. (total: 61,651 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS monomers

NRPS/PKS monomer predictions

scaffold_5 - Region 5 - T1PKS

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Location: 2,296,090 - 2,363,523 nt. (total: 67,434 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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No matches found.

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Predicted core structure(s)

For candidate cluster 8, location 2296089 - 2363523:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

419663

PKS_AT (550..854): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 90.4
Methoxymalonyl-CoA: 75.8
Malonyl-CoA: 47.8
Isobutyryl-CoA: 38.4
Propionyl-CoA: 36.2
2-Methylbutyryl-CoA: 28.2
CHC-CoA: 25.7
fatty_acid: 22.3
3-Methylbutyryl-CoA: 21.3
Benzoyl-CoA: 19.2
inactive: 16.7
trans-1,2-CPDA: 15.3
Acetyl-CoA: 15.2
Ethylmalonyl-CoA: -4.2

scaffold_6 - Region 1 - T1PKS

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Location: 229,778 - 292,475 nt. (total: 62,698 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 229777 - 292475:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

527279

PKS_AT (556..828): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 65.7
inactive: 44.2
Methoxymalonyl-CoA: 35.7
Methylmalonyl-CoA: 24.7
Benzoyl-CoA: 18.5
Propionyl-CoA: 15.0
Acetyl-CoA: 13.2
fatty_acid: 12.5
2-Methylbutyryl-CoA: 11.0
Isobutyryl-CoA: 10.9
Ethylmalonyl-CoA: 10.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_6 - Region 2 - terpene

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Location: 1,026,566 - 1,057,658 nt. (total: 31,093 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_6 - Region 3 - T1PKS

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Location: 1,393,339 - 1,463,126 nt. (total: 69,788 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 3, location 1393338 - 1463126:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

380069

PKS_AT (910..1206): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 131.1
Methylmalonyl-CoA: 76.0
inactive: 70.1
Methoxymalonyl-CoA: 66.5
Ethylmalonyl-CoA: 44.8
Propionyl-CoA: 39.3
2-Methylbutyryl-CoA: 28.6
Isobutyryl-CoA: 27.7
CHC-CoA: 21.1
Benzoyl-CoA: 18.7
fatty_acid: 17.5
3-Methylbutyryl-CoA: 16.9
Acetyl-CoA: 13.4
trans-1,2-CPDA: 10.4

scaffold_6 - Region 4 - NRPS-like

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Location: 2,073,771 - 2,137,015 nt. (total: 63,245 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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No matches found.

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 4, location 2073770 - 2137015:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

420954

AMP-binding (18..335): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---LATI--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_7 - Region 1 - T1PKS

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Location: 242,762 - 305,690 nt. (total: 62,929 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 242761 - 305690:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

256443

PKS_AT (589..882): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; CE-Malonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 114.8
Ethylmalonyl-CoA: 91.6
Methoxymalonyl-CoA: 88.6
Malonyl-CoA: 74.0
Isobutyryl-CoA: 58.4
Propionyl-CoA: 56.9
2-Methylbutyryl-CoA: 53.0
Acetyl-CoA: 49.9
fatty_acid: 43.5
trans-1,2-CPDA: 35.1
Benzoyl-CoA: 33.7
3-Methylbutyryl-CoA: 31.4
CHC-CoA: 21.8
inactive: 14.9

scaffold_7 - Region 2 - indole

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Location: 441,744 - 473,054 nt. (total: 31,311 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_7 - Region 3 - T1PKS

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Location: 1,414,029 - 1,479,632 nt. (total: 65,604 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1414028 - 1479632:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

267363

Search NORINE for peptide: strict or relaxed

AMP-binding (60..381): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: AYHFFCQQVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

466026

PKS_AT (571..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 83.5
Methylmalonyl-CoA: 77.2
Methoxymalonyl-CoA: 72.4
Ethylmalonyl-CoA: 44.0
Isobutyryl-CoA: 34.5
trans-1,2-CPDA: 30.2
Propionyl-CoA: 29.8
Benzoyl-CoA: 28.3
inactive: 25.0
2-Methylbutyryl-CoA: 21.6
fatty_acid: 14.3
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_7 - Region 4 - T1PKS

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Location: 1,845,523 - 1,912,928 nt. (total: 67,406 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 4, location 1845522 - 1912928:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

270979

PKS_AT (600..913): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.3
Methoxymalonyl-CoA: 57.9
Malonyl-CoA: 51.0
Benzoyl-CoA: 28.2
Ethylmalonyl-CoA: 27.8
Propionyl-CoA: 25.9
3-Methylbutyryl-CoA: 16.1
fatty_acid: 15.5
inactive: 12.1
CHC-CoA: 11.0
2-Methylbutyryl-CoA: 6.1
Isobutyryl-CoA: 4.8
trans-1,2-CPDA: 0.0
Acetyl-CoA: -0.2

scaffold_7 - Region 5 - fungal-RiPP-like

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Location: 1,987,936 - 2,072,994 nt. (total: 85,059 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_7 - Region 6 - fungal-RiPP-like

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Location: 2,158,589 - 2,249,452 nt. (total: 90,864 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_7 - Region 7 - terpene

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Location: 2,264,802 - 2,296,281 nt. (total: 31,480 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_8 - Region 1 - fungal-RiPP-like

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Location: 6,971 - 67,261 nt. (total: 60,291 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_8 - Region 2 - NRPS-like,fungal-RiPP-like

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Location: 294,824 - 433,907 nt. (total: 139,084 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
fungal-RiPP-like: DUF3328

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Predicted core structure(s)

For candidate cluster 2, location 294823 - 433907:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 294823 - 349292:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

530288

AMP-binding (29..331): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AHSYMGHQIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_8 - Region 3 - NRPS-like

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Location: 847,780 - 906,327 nt. (total: 58,548 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 5, location 847779 - 906327:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

536260

AMP-binding (51..486): X

nrpys: 4-ohPpa

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VAVFAGGAGK
Nearest Stachelhaus code(s):: 4-ohPpa VAVFAGGAGK (79% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_8 - Region 4 - NRPS,T1PKS

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Location: 913,712 - 985,085 nt. (total: 71,374 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 6, location 913711 - 985085:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

489694

PKS_AT (559..872): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 80.8
Methoxymalonyl-CoA: 73.8
Ethylmalonyl-CoA: 57.2
Isobutyryl-CoA: 42.3
Malonyl-CoA: 41.0
trans-1,2-CPDA: 40.5
2-Methylbutyryl-CoA: 35.8
Propionyl-CoA: 32.1
Benzoyl-CoA: 28.7
fatty_acid: 27.6
CHC-CoA: 22.6
3-Methylbutyryl-CoA: 15.1
Acetyl-CoA: 14.7
inactive: 0.0

AMP-binding (3115..3515): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFDRWGSTCK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_8 - Region 5 - T1PKS

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Location: 1,109,952 - 1,175,396 nt. (total: 65,445 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 7, location 1109951 - 1175396:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

284244

PKS_AT (592..901): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; Isobutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 79.6
Methoxymalonyl-CoA: 63.0
Malonyl-CoA: 51.3
Ethylmalonyl-CoA: 48.5
Propionyl-CoA: 37.4
Isobutyryl-CoA: 32.6
2-Methylbutyryl-CoA: 26.0
CHC-CoA: 19.0
trans-1,2-CPDA: 14.1
Benzoyl-CoA: 13.2
Acetyl-CoA: 11.9
fatty_acid: 7.9
inactive: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_8 - Region 6 - T1PKS,fungal-RiPP-like

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Location: 1,178,172 - 1,354,467 nt. (total: 176,296 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 8, location 1178171 - 1354467:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 10, location 1258075 - 1326134:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

402927

PKS_AT (527..835): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 60.7
Methoxymalonyl-CoA: 58.1
Ethylmalonyl-CoA: 35.7
Malonyl-CoA: 33.9
Propionyl-CoA: 30.4
Isobutyryl-CoA: 19.0
trans-1,2-CPDA: 18.9
Benzoyl-CoA: 18.2
fatty_acid: 15.4
inactive: 14.1
2-Methylbutyryl-CoA: 3.1
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_8 - Region 7 - NRPS-like

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Location: 2,039,746 - 2,102,643 nt. (total: 62,898 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 12, location 2039745 - 2102643:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

531384

AMP-binding (73..473): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VATFIGEAGK
Nearest Stachelhaus code(s):: Ppa VATFIGGAGK (82% 8� match)
Stachelhaus code match:: 90% (moderate)

scaffold_9 - Region 1 - NRP-metallophore,NRPS,fungal-RiPP-like

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Location: 73 - 105,598 nt. (total: 105,526 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))
fungal-RiPP-like: DUF3328

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NRPS/PKS monomers

NRPS/PKS monomer predictions

403420

Search NORINE for peptide: strict or relaxed

AMP-binding (257..695): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPMMWMFINK
Nearest Stachelhaus code(s):: Aad DPMMWMAINK (79% 8� match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1401..1808): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQHTITVVK
Nearest Stachelhaus code(s):: Cys DVQHTITVVK (82% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2513..2920): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVGIAGAIGK
Nearest Stachelhaus code(s):: E-ahmohOrn DVGGGGVIGK (68% 8� match); Arg DVENVGAIGK (50% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_9 - Region 2 - fungal-RiPP-like

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Location: 1,182,337 - 1,273,504 nt. (total: 91,168 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_9 - Region 3 - NRPS-like

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Location: 1,404,781 - 1,460,638 nt. (total: 55,858 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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Module view

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 1404780 - 1460638:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

306935

AMP-binding (34..366): X

nrpys: Gly, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGASGGRFLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_9 - Region 4 - NRPS-like

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Location: 1,933,095 - 1,990,915 nt. (total: 57,821 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

471209

AMP-binding (23..343): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AATIAVN--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)