Cladonia grayi Cgr/DA2myc/ss v2.0

Identified secondary metabolite regions using strictness 'relaxed'

scaffold_00003

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS-like	589,275	653,170

scaffold_00004

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	terpene	25,258	57,013

scaffold_00005

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	T1PKS,fungal-RiPP-like	2,961	93,989
Region 5.2	T1PKS	402,323	469,817

scaffold_00006

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	T1PKS	49,331	105,365
Region 6.2	T1PKS	180,125	265,826	tricholignan A	Polyketide:Iterative type I polyketide	22%

scaffold_00007

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS-like	370,494	434,945
Region 7.2	other	464,853	525,761
Region 7.3	T1PKS	545,284	612,114	agnestin A/agnestin B	Polyketide	14%

scaffold_00008

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	terpene	96,727	128,808
Region 8.2	T1PKS	299,480	366,464	6-hydroxymellein	Polyketide	100%

scaffold_00010

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	terpene	317,071	349,045
Region 10.2	T3PKS,T1PKS	362,135	432,337

scaffold_00011

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	fungal-RiPP-like	456,638	508,868	usnic acid	Polyketide	20%

scaffold_00013

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	T1PKS	1	47,813	grayanic acid	Polyketide	100%

scaffold_00015

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	T1PKS,terpene	5,768	86,089
Region 15.2	terpene	153,650	185,318	squalestatin S1	Terpene	40%

scaffold_00016

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	NRPS	24,631	93,225	peramine	NRP	100%

scaffold_00017

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	NRPS-like	186,279	249,056	aspulvinone H/aspulvinone B1	NRP	100%

scaffold_00019

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	T3PKS	150,740	212,108

scaffold_00023

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	terpene	74,947	106,159

scaffold_00024

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	fungal-RiPP-like	180,672	277,188

scaffold_00025

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	T1PKS	1	48,487
Region 25.2	NRPS-like	85,351	149,583

scaffold_00027

Region	Type	From	To	Most similar known cluster		Similarity
Region 27.1	indole	77,959	109,394	biscognienyne B/eutypinic acid/compound 3/4-hydroxy-3-prenylbenzoic acid/prenylhydroquinone/compound 15/compound 8/compound 7/compound 6/compound 11/compound 12/pestalodiol E	Other	63%

scaffold_00030

Region	Type	From	To	Most similar known cluster		Similarity
Region 30.1	T1PKS	151,006	217,829	scytalone/T3HN	Polyketide	40%

scaffold_00032

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	NRPS-like	239,098	290,970

scaffold_00035

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	fungal-RiPP-like	172,555	266,185

scaffold_00036

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	NRPS-like	1	38,126
Region 36.2	T1PKS	183,672	251,976

scaffold_00038

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	T1PKS	211,702	262,756	terreic acid	Polyketide	55%

scaffold_00039

Region	Type	From	To	Most similar known cluster		Similarity
Region 39.1	NRPS-like	181,215	244,827

scaffold_00049

Region	Type	From	To	Most similar known cluster		Similarity
Region 49.1	NRPS-like	73,351	136,544

scaffold_00050

Region	Type	From	To	Most similar known cluster		Similarity
Region 50.1	T1PKS	83,679	150,716

scaffold_00059

Region	Type	From	To	Most similar known cluster		Similarity
Region 59.1	fungal-RiPP-like	126,522	182,048

scaffold_00063

Region	Type	From	To	Most similar known cluster		Similarity
Region 63.1	T1PKS	9,334	77,683	trichoxide	Polyketide	33%

scaffold_00065

Region	Type	From	To	Most similar known cluster		Similarity
Region 65.1	T1PKS,fungal-RiPP-like	1	147,630

scaffold_00073

Region	Type	From	To	Most similar known cluster		Similarity
Region 73.1	T3PKS	91,927	138,635

scaffold_00075

Region	Type	From	To	Most similar known cluster		Similarity
Region 75.1	NRPS-like	1	49,511

scaffold_00082

Region	Type	From	To	Most similar known cluster		Similarity
Region 82.1	NRPS-like	1	51,347	aspulvinone H/aspulvinone B1	NRP	100%

scaffold_00083

Region	Type	From	To	Most similar known cluster		Similarity
Region 83.1	fungal-RiPP-like	1	74,970

scaffold_00084

Region	Type	From	To	Most similar known cluster		Similarity
Region 84.1	fungal-RiPP-like	145	91,174

scaffold_00085

Region	Type	From	To	Most similar known cluster		Similarity
Region 85.1	fungal-RiPP-like	1	80,696

scaffold_00088

Region	Type	From	To	Most similar known cluster		Similarity
Region 88.1	NRPS-like	1	43,248

scaffold_00093

Region	Type	From	To	Most similar known cluster		Similarity
Region 93.1	fungal-RiPP-like	1	76,770	clavaric acid	Terpene	100%

scaffold_00096

Region	Type	From	To	Most similar known cluster		Similarity
Region 96.1	NRPS-like	1	61,130

scaffold_00104

Region	Type	From	To	Most similar known cluster		Similarity
Region 104.1	T1PKS,NRPS	1	41,343

scaffold_00106

Region	Type	From	To	Most similar known cluster		Similarity
Region 106.1	T1PKS	33,971	95,241

scaffold_00121

Region	Type	From	To	Most similar known cluster		Similarity
Region 121.1	NRPS-like	1	54,450

scaffold_00126

Region	Type	From	To	Most similar known cluster		Similarity
Region 126.1	T1PKS	636	64,029	sordarial	Polyketide	50%

scaffold_00128

Region	Type	From	To	Most similar known cluster		Similarity
Region 128.1	T1PKS	30,914	77,948

scaffold_00139

Region	Type	From	To	Most similar known cluster		Similarity
Region 139.1	fungal-RiPP-like	1	55,502

scaffold_00140

Region	Type	From	To	Most similar known cluster		Similarity
Region 140.1	T1PKS	1	46,389	scytalone/T3HN	Polyketide	40%

scaffold_00143

Region	Type	From	To	Most similar known cluster		Similarity
Region 143.1	fungal-RiPP-like,NRPS-like	1	65,775

scaffold_00144

Region	Type	From	To	Most similar known cluster		Similarity
Region 144.1	T1PKS	1	64,409

scaffold_00156

Region	Type	From	To	Most similar known cluster		Similarity
Region 156.1	T1PKS	1	51,445

scaffold_00172

Region	Type	From	To	Most similar known cluster		Similarity
Region 171.1	fungal-RiPP-like	1	43,382

scaffold_00179

Region	Type	From	To	Most similar known cluster		Similarity
Region 178.1	fungal-RiPP-like	1	41,025

scaffold_00182

Region	Type	From	To	Most similar known cluster		Similarity
Region 181.1	T1PKS	1	39,236

scaffold_00202

Region	Type	From	To	Most similar known cluster		Similarity
Region 201.1	NRPS	1	31,837

scaffold_00244

Region	Type	From	To	Most similar known cluster		Similarity
Region 243.1	fungal-RiPP-like	1	18,807

No secondary metabolite regions were found in these records:: scaffold_00001; scaffold_00002; scaffold_00009; scaffold_00012; scaffold_00014; scaffold_00018; scaffold_00020; scaffold_00021; scaffold_00022; scaffold_00026; scaffold_00028; scaffold_00029; scaffold_00031; scaffold_00033; scaffold_00034; scaffold_00037; scaffold_00040; scaffold_00041; scaffold_00042; scaffold_00043; scaffold_00044; scaffold_00045; scaffold_00046; scaffold_00047; scaffold_00048; scaffold_00051; scaffold_00052; scaffold_00053; scaffold_00054; scaffold_00055; scaffold_00056; scaffold_00057; scaffold_00058; scaffold_00060; scaffold_00061; scaffold_00062; scaffold_00064; scaffold_00066; scaffold_00067; scaffold_00068; scaffold_00069; scaffold_00070; scaffold_00071; scaffold_00072; scaffold_00074; scaffold_00076; scaffold_00077; scaffold_00078; scaffold_00079; scaffold_00080; scaffold_00081; scaffold_00086; scaffold_00087; scaffold_00089; scaffold_00090; scaffold_00091; scaffold_00092; scaffold_00094; scaffold_00095; scaffold_00097; scaffold_00098; scaffold_00099; scaffold_00100; scaffold_00101; scaffold_00102; scaffold_00103; scaffold_00105; scaffold_00107; scaffold_00108; scaffold_00109; scaffold_00110; scaffold_00111; scaffold_00112; scaffold_00113; scaffold_00114; scaffold_00115; scaffold_00116; scaffold_00117; scaffold_00118; scaffold_00119; scaffold_00120; scaffold_00122; scaffold_00123; scaffold_00124; scaffold_00125; scaffold_00127; scaffold_00129; scaffold_00130; scaffold_00131; scaffold_00132; scaffold_00133; scaffold_00134; scaffold_00135; scaffold_00136; scaffold_00137; scaffold_00138; scaffold_00141; scaffold_00142; scaffold_00145; scaffold_00146; scaffold_00147; scaffold_00148; scaffold_00149; scaffold_00150; scaffold_00151; scaffold_00152; scaffold_00153; scaffold_00154; scaffold_00155; scaffold_00157; scaffold_00158; scaffold_00160; scaffold_00161; scaffold_00162; scaffold_00163; scaffold_00164; scaffold_00165; scaffold_00166; scaffold_00167; scaffold_00168; scaffold_00169; scaffold_00170; scaffold_00171; scaffold_00173; scaffold_00174; scaffold_00175; scaffold_00176; scaffold_00177; scaffold_00178; scaffold_00180; scaffold_00181; scaffold_00183; scaffold_00184; scaffold_00185; scaffold_00186; scaffold_00187; scaffold_00188; scaffold_00189; scaffold_00190; scaffold_00191; scaffold_00192; scaffold_00193; scaffold_00194; scaffold_00195; scaffold_00196; scaffold_00197; scaffold_00198; scaffold_00199; scaffold_00200; scaffold_00201; scaffold_00203; scaffold_00204; scaffold_00205; scaffold_00206; scaffold_00207; scaffold_00208; scaffold_00209; scaffold_00210; scaffold_00211; scaffold_00212; scaffold_00213; scaffold_00214; scaffold_00215; scaffold_00216; scaffold_00217; scaffold_00218; scaffold_00219; scaffold_00220; scaffold_00221; scaffold_00222; scaffold_00223; scaffold_00224; scaffold_00225; scaffold_00226; scaffold_00227; scaffold_00228; scaffold_00229; scaffold_00230; scaffold_00231; scaffold_00232; scaffold_00233; scaffold_00234; scaffold_00235; scaffold_00236; scaffold_00237; scaffold_00238; scaffold_00239; scaffold_00240; scaffold_00241; scaffold_00242; scaffold_00243; scaffold_00245; scaffold_00246; scaffold_00247; scaffold_00248; scaffold_00249; scaffold_00250; scaffold_00251; scaffold_00252; scaffold_00253; scaffold_00254; scaffold_00255; scaffold_00256; scaffold_00257; scaffold_00258; scaffold_00259; scaffold_00260; scaffold_00261; scaffold_00262; scaffold_00263; scaffold_00264; scaffold_00265; scaffold_00266; scaffold_00267; scaffold_00268; scaffold_00269; scaffold_00270; scaffold_00271; scaffold_00272; scaffold_00273; scaffold_00274; scaffold_00275; scaffold_00276; scaffold_00277; scaffold_00278; scaffold_00279; scaffold_00280; scaffold_00281; scaffold_00282; scaffold_00283; scaffold_00284; scaffold_00285; scaffold_00286; scaffold_00287; scaffold_00288; scaffold_00289; scaffold_00290; scaffold_00291; scaffold_00292; scaffold_00293; scaffold_00294; scaffold_00295; scaffold_00296; scaffold_00297; scaffold_00298; scaffold_00299; scaffold_00300; scaffold_00301; scaffold_00302; scaffold_00303; scaffold_00304; scaffold_00305; scaffold_00306; scaffold_00307; scaffold_00308; scaffold_00309; scaffold_00310; scaffold_00311; scaffold_00312; scaffold_00313; scaffold_00314; scaffold_00315; scaffold_00316; scaffold_00317; scaffold_00318; scaffold_00319; scaffold_00320; scaffold_00321; scaffold_00322; scaffold_00323; scaffold_00324; scaffold_00325; scaffold_00326; scaffold_00327; scaffold_00328; scaffold_00329; scaffold_00330; scaffold_00331; scaffold_00332; scaffold_00333; scaffold_00334; scaffold_00335; scaffold_00336; scaffold_00337; scaffold_00338; scaffold_00339; scaffold_00340; scaffold_00341; scaffold_00342; scaffold_00343; scaffold_00344; scaffold_00345; scaffold_00346; scaffold_00347; scaffold_00348; scaffold_00349; scaffold_00350; scaffold_00351; scaffold_00352; scaffold_00353; scaffold_00354; scaffold_00355; scaffold_00356; scaffold_00357; scaffold_00358; scaffold_00359; scaffold_00360; scaffold_00361; scaffold_00362; scaffold_00363; scaffold_00364; scaffold_00365; scaffold_00366; scaffold_00367; scaffold_00368; scaffold_00369; scaffold_00370; scaffold_00371; scaffold_00372; scaffold_00373; scaffold_00374; scaffold_00375; scaffold_00376; scaffold_00377; scaffold_00378; scaffold_00379; scaffold_00380; scaffold_00381; scaffold_00382; scaffold_00383; scaffold_00384; scaffold_00385; scaffold_00386; scaffold_00387; scaffold_00388; scaffold_00389; scaffold_00390; scaffold_00391; scaffold_00392; scaffold_00393; scaffold_00394; scaffold_00395; scaffold_00396; scaffold_00397; scaffold_00398; scaffold_00399; scaffold_00400; scaffold_00401; scaffold_00402; scaffold_00403; scaffold_00404; scaffold_00405; scaffold_00406; scaffold_00407; scaffold_00408; scaffold_00409; scaffold_00410; scaffold_00411; scaffold_00412; scaffold_00413; scaffold_00414; scaffold_00415

Compact view

scaffold_00003 - Region 1 - NRPS-like

Download region GenBank file

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Location: 589,275 - 653,170 nt. (total: 63,896 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 589274 - 653170:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

850

AMP-binding (19..341): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIPSCSLF-K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00004 - Region 1 - terpene

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Location: 25,258 - 57,013 nt. (total: 31,756 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_00005 - Region 1 - T1PKS,fungal-RiPP-like

Download region GenBank file

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Location: 2,961 - 93,989 nt. (total: 91,029 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 2960 - 93989:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 2960 - 38213:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1155

PKS_AT (533..839): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; 2-Methylbutyryl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 89.8
Methoxymalonyl-CoA: 78.1
Ethylmalonyl-CoA: 59.4
Isobutyryl-CoA: 57.5
Malonyl-CoA: 54.6
Propionyl-CoA: 44.9
Benzoyl-CoA: 39.5
fatty_acid: 37.2
trans-1,2-CPDA: 32.9
2-Methylbutyryl-CoA: 28.4
CHC-CoA: 24.4
inactive: 13.6
3-Methylbutyryl-CoA: 12.9
Acetyl-CoA: 10.7

scaffold_00005 - Region 2 - T1PKS

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Location: 402,323 - 469,817 nt. (total: 67,495 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

1319

PKS_AT (568..881): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; 2-Methylbutyryl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 46.3
Methoxymalonyl-CoA: 37.8
Propionyl-CoA: 24.6
Malonyl-CoA: 24.2
Isobutyryl-CoA: 12.4
3-Methylbutyryl-CoA: 10.9
inactive: 10.5
2-Methylbutyryl-CoA: 10.1
Ethylmalonyl-CoA: 9.6
Benzoyl-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_00006 - Region 1 - T1PKS

Download region GenBank file

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Location: 49,331 - 105,365 nt. (total: 56,035 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 49330 - 105365:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1417

PKS_AT (539..841): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 72.1
Malonyl-CoA: 67.0
Methoxymalonyl-CoA: 62.8
Isobutyryl-CoA: 47.3
Ethylmalonyl-CoA: 39.4
Propionyl-CoA: 27.3
Benzoyl-CoA: 25.3
inactive: 17.2
fatty_acid: 9.9
trans-1,2-CPDA: 9.7
3-Methylbutyryl-CoA: 7.7
2-Methylbutyryl-CoA: 5.5
CHC-CoA: 0.0
Acetyl-CoA: 0.0

scaffold_00006 - Region 2 - T1PKS

Download region GenBank file

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Location: 180,125 - 265,826 nt. (total: 85,702 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 180124 - 265826:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 1474 + 1470

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1470

PKS_AT (906..1206): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.8
Methoxymalonyl-CoA: 47.8
Methylmalonyl-CoA: 42.3
inactive: 37.4
Isobutyryl-CoA: 20.7
Benzoyl-CoA: 17.2
Propionyl-CoA: 12.3
fatty_acid: 9.7
Acetyl-CoA: 9.5
Ethylmalonyl-CoA: 8.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

1474

PKS_AT (611..925): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 81.8
Methoxymalonyl-CoA: 70.4
Malonyl-CoA: 68.3
Isobutyryl-CoA: 55.4
Ethylmalonyl-CoA: 51.7
2-Methylbutyryl-CoA: 42.8
Propionyl-CoA: 34.8
Benzoyl-CoA: 25.2
trans-1,2-CPDA: 24.4
3-Methylbutyryl-CoA: 18.4
fatty_acid: 16.7
CHC-CoA: 16.6
Acetyl-CoA: 14.3
inactive: 13.3

scaffold_00007 - Region 1 - NRPS-like

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Location: 370,494 - 434,945 nt. (total: 64,452 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 370493 - 434945:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Aad)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1747

Search NORINE for peptide: strict or relaxed

AMP-binding (33..510): Aad

nrpys: Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFVMRAK
Nearest Stachelhaus code(s):: Aad DPRHFVMRAK (97% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_00007 - Region 2 - other

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Location: 464,853 - 525,761 nt. (total: 60,909 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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scaffold_00007 - Region 3 - T1PKS

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Location: 545,284 - 612,114 nt. (total: 66,831 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 3, location 545283 - 612114:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1822

PKS_AT (909..1205): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 118.1
Methylmalonyl-CoA: 73.0
inactive: 71.5
Methoxymalonyl-CoA: 63.7
Propionyl-CoA: 50.1
Isobutyryl-CoA: 30.4
3-Methylbutyryl-CoA: 26.1
Ethylmalonyl-CoA: 23.7
Acetyl-CoA: 21.1
2-Methylbutyryl-CoA: 20.5
CHC-CoA: 19.5
Benzoyl-CoA: 17.0
trans-1,2-CPDA: 13.4
fatty_acid: 12.4

scaffold_00008 - Region 1 - terpene

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Location: 96,727 - 128,808 nt. (total: 32,082 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_00008 - Region 2 - T1PKS

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Location: 299,480 - 366,464 nt. (total: 66,985 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 299479 - 366464:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

1944

PKS_AT (908..1206): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 91.8
inactive: 55.8
Methoxymalonyl-CoA: 47.5
Methylmalonyl-CoA: 45.3
fatty_acid: 26.1
Propionyl-CoA: 17.5
2-Methylbutyryl-CoA: 17.4
Benzoyl-CoA: 15.5
3-Methylbutyryl-CoA: 15.2
Ethylmalonyl-CoA: 14.9
Isobutyryl-CoA: 12.3
Acetyl-CoA: 12.3
CHC-CoA: 10.4
trans-1,2-CPDA: 8.8

scaffold_00010 - Region 1 - terpene

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Location: 317,071 - 349,045 nt. (total: 31,975 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_00010 - Region 2 - T1PKS,T3PKS

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Location: 362,135 - 432,337 nt. (total: 70,203 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 2, location 362134 - 432337:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 362134 - 423497:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 363991 - 432337:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

2367

PKS_AT (612..916): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 72.1
Methoxymalonyl-CoA: 57.1
Malonyl-CoA: 45.5
Isobutyryl-CoA: 44.6
Ethylmalonyl-CoA: 44.2
Propionyl-CoA: 30.7
fatty_acid: 30.6
Benzoyl-CoA: 23.1
trans-1,2-CPDA: 20.3
CHC-CoA: 10.3
2-Methylbutyryl-CoA: 8.0
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00011 - Region 1 - fungal-RiPP-like

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Location: 456,638 - 508,868 nt. (total: 52,231 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00013 - Region 1 - T1PKS

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Location: 1 - 47,813 nt. (total: 47,813 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 47813:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

2764

PKS_AT (888..1191): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 54.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 94.9
Methoxymalonyl-CoA: 61.0
Methylmalonyl-CoA: 59.2
inactive: 57.2
Propionyl-CoA: 29.5
Ethylmalonyl-CoA: 22.2
2-Methylbutyryl-CoA: 22.1
Benzoyl-CoA: 19.5
Acetyl-CoA: 15.6
CHC-CoA: 15.3
Isobutyryl-CoA: 13.3
3-Methylbutyryl-CoA: 13.3
fatty_acid: 11.9
trans-1,2-CPDA: 10.5

scaffold_00015 - Region 1 - T1PKS,terpene

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Location: 5,768 - 86,089 nt. (total: 80,322 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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No matches found.

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 5767 - 86089:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 5767 - 73401:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

3082

PKS_AT (906..1197): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 49.8
inactive: 46.1
Malonyl-CoA: 46.1
Methylmalonyl-CoA: 42.3
Ethylmalonyl-CoA: 27.3
Propionyl-CoA: 25.8
CHC-CoA: 24.0
2-Methylbutyryl-CoA: 21.4
trans-1,2-CPDA: 16.4
Isobutyryl-CoA: 16.1
Benzoyl-CoA: 15.3
Acetyl-CoA: 14.7
fatty_acid: 13.6
3-Methylbutyryl-CoA: 10.0

scaffold_00015 - Region 2 - terpene

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Location: 153,650 - 185,318 nt. (total: 31,669 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_00016 - Region 1 - NRPS

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Location: 24,631 - 93,225 nt. (total: 68,595 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 24630 - 93225:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

3221

Search NORINE for peptide: strict or relaxed

AMP-binding (333..732): X

nrpys: Pyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVHHITEINK
Nearest Stachelhaus code(s):: Pyr DVHHITEINK (91% 8� match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1407..1808): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSDTGAPTK
Nearest Stachelhaus code(s):: Arg DVSDTGAPTK (97% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_00017 - Region 1 - NRPS-like

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Location: 186,279 - 249,056 nt. (total: 62,778 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 186278 - 249056:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

3425

AMP-binding (31..435): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VALFTGGAGK
Nearest Stachelhaus code(s):: 4-ohPpa VALFNGGAGK (71% 8� match)
Stachelhaus code match:: 90% (moderate)

scaffold_00019 - Region 1 - T3PKS

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Location: 150,740 - 212,108 nt. (total: 61,369 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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scaffold_00023 - Region 1 - terpene

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Location: 74,947 - 106,159 nt. (total: 31,213 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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scaffold_00024 - Region 1 - fungal-RiPP-like

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Location: 180,672 - 277,188 nt. (total: 96,517 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_00025 - Region 1 - T1PKS

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Location: 1 - 48,487 nt. (total: 48,487 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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No matches found.

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 48487:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

4352

PKS_AT (241..546): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 54.2
Methylmalonyl-CoA: 49.1
Malonyl-CoA: 27.5
Isobutyryl-CoA: 25.8
Ethylmalonyl-CoA: 21.9
trans-1,2-CPDA: 16.7
fatty_acid: 14.2
Propionyl-CoA: 10.4
2-Methylbutyryl-CoA: 8.5
Benzoyl-CoA: 8.4
inactive: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00025 - Region 2 - NRPS-like

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Location: 85,351 - 149,583 nt. (total: 64,233 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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No matches found.

NRPS/PKS monomers

NRPS/PKS monomer predictions

4394

AMP-binding (255..661): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVIIAGIICK
Nearest Stachelhaus code(s):: Gln DVQIIGGIHK (59% 8� match); Trp or Pro DVYFVGGICK (53% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_00027 - Region 1 - indole

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Location: 77,959 - 109,394 nt. (total: 31,436 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

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scaffold_00030 - Region 1 - T1PKS

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Location: 151,006 - 217,829 nt. (total: 66,824 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 151005 - 217829:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

4946

PKS_AT (913..1210): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 62.5%; Methoxymalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 105.9
inactive: 73.5
Methoxymalonyl-CoA: 51.3
Methylmalonyl-CoA: 46.3
Propionyl-CoA: 30.6
3-Methylbutyryl-CoA: 19.4
Isobutyryl-CoA: 17.5
fatty_acid: 14.9
CHC-CoA: 14.5
2-Methylbutyryl-CoA: 14.1
Benzoyl-CoA: 13.5
Ethylmalonyl-CoA: 8.3
Acetyl-CoA: 7.5
trans-1,2-CPDA: 0.0

scaffold_00032 - Region 1 - NRPS-like

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Location: 239,098 - 290,970 nt. (total: 51,873 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

NRPS/PKS monomer predictions

5196

AMP-binding (23..431): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TLPVMGWTPK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00035 - Region 1 - fungal-RiPP-like

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Location: 172,555 - 266,185 nt. (total: 93,631 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_00036 - Region 1 - NRPS-like

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Location: 1 - 38,126 nt. (total: 38,126 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 38126:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

5489

AMP-binding (306..701): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVMLAGCACK
Nearest Stachelhaus code(s):: Leu DAMLVGAACK (50% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_00036 - Region 2 - T1PKS

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Location: 183,672 - 251,976 nt. (total: 68,305 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 183671 - 251976:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

5566

PKS_AT (601..917): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 88.7
Methoxymalonyl-CoA: 85.0
Malonyl-CoA: 60.7
Isobutyryl-CoA: 57.9
Ethylmalonyl-CoA: 57.1
Propionyl-CoA: 29.8
Benzoyl-CoA: 29.2
2-Methylbutyryl-CoA: 27.0
trans-1,2-CPDA: 23.3
inactive: 18.5
3-Methylbutyryl-CoA: 15.6
CHC-CoA: 15.4
fatty_acid: 12.5
Acetyl-CoA: 10.1

scaffold_00038 - Region 1 - T1PKS

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Location: 211,702 - 262,756 nt. (total: 51,055 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 211701 - 262756:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk)
Non-colinear ordering:: 5768 + 5767

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

5768

PKS_AT (545..748): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 59.0
Methoxymalonyl-CoA: 58.1
Malonyl-CoA: 40.8
Ethylmalonyl-CoA: 37.2
Propionyl-CoA: 27.9
fatty_acid: 23.8
3-Methylbutyryl-CoA: 22.5
Benzoyl-CoA: 20.1
2-Methylbutyryl-CoA: 19.9
CHC-CoA: 18.2
Acetyl-CoA: 17.5
trans-1,2-CPDA: 13.9
inactive: -2.2

scaffold_00039 - Region 1 - NRPS-like

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Location: 181,215 - 244,827 nt. (total: 63,613 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 181214 - 244827:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

5840

AMP-binding (41..377): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGASGGRFLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00049 - Region 1 - NRPS-like

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Location: 73,351 - 136,544 nt. (total: 63,194 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 73350 - 136544:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

6679

AMP-binding (24..329): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SAFILGLLLK
Nearest Stachelhaus code(s):: Hpg DAFHLGLLCK (38% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_00050 - Region 1 - T1PKS

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Location: 83,679 - 150,716 nt. (total: 67,038 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 83678 - 150716:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

6765

PKS_AT (565..862): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 90.7
Methoxymalonyl-CoA: 75.0
Isobutyryl-CoA: 72.5
Malonyl-CoA: 60.0
Ethylmalonyl-CoA: 51.1
Propionyl-CoA: 40.1
Benzoyl-CoA: 30.6
trans-1,2-CPDA: 30.2
3-Methylbutyryl-CoA: 29.0
inactive: 25.7
2-Methylbutyryl-CoA: 21.2
CHC-CoA: 18.2
fatty_acid: 16.0
Acetyl-CoA: 12.3

scaffold_00059 - Region 1 - fungal-RiPP-like

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Location: 126,522 - 182,048 nt. (total: 55,527 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00063 - Region 1 - T1PKS

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Location: 9,334 - 77,683 nt. (total: 68,350 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 9333 - 77683:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

7652

PKS_AT (596..920): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Isobutyryl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 68.2
Methoxymalonyl-CoA: 50.5
Malonyl-CoA: 44.1
Propionyl-CoA: 29.1
trans-1,2-CPDA: 20.9
Ethylmalonyl-CoA: 19.7
Isobutyryl-CoA: 18.2
fatty_acid: 17.8
CHC-CoA: 17.1
Benzoyl-CoA: 12.9
2-Methylbutyryl-CoA: 6.9
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00065 - Region 1 - T1PKS,fungal-RiPP-like

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Location: 1 - 147,630 nt. (total: 147,630 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
fungal-RiPP-like: DUF3328

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 147630:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 65832:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

7773

PKS_AT (887..1182): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.8
inactive: 63.7
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 41.5
Benzoyl-CoA: 17.0
fatty_acid: 13.7
Isobutyryl-CoA: 12.8
2-Methylbutyryl-CoA: 12.1
Acetyl-CoA: 11.2
Ethylmalonyl-CoA: 10.9
Propionyl-CoA: 10.7
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00073 - Region 1 - T3PKS

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Location: 91,927 - 138,635 nt. (total: 46,709 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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scaffold_00075 - Region 1 - NRPS-like

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Location: 1 - 49,511 nt. (total: 49,511 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 49511:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

8307

AMP-binding (1..236): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GGRYCASPIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00082 - Region 1 - NRPS-like

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Location: 1 - 51,347 nt. (total: 51,347 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 51347:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

8652

AMP-binding (46..457): X

nrpys: 4-ohPpa

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VAVFAGGAGK
Nearest Stachelhaus code(s):: 4-ohPpa VAVFAGGAGK (82% 8� match)
Stachelhaus code match:: 100% (strong)

scaffold_00083 - Region 1 - fungal-RiPP-like

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Location: 1 - 74,970 nt. (total: 74,970 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00084 - Region 1 - fungal-RiPP-like

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Location: 145 - 91,174 nt. (total: 91,030 nt) Show pHMM detection rules used

fungal-RiPP-like: DUF3328

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scaffold_00085 - Region 1 - fungal-RiPP-like

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Location: 1 - 80,696 nt. (total: 80,696 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00088 - Region 1 - NRPS-like

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Location: 1 - 43,248 nt. (total: 43,248 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 43248:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

8896

AMP-binding (44..325): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -FLCFTM--K
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00093 - Region 1 - fungal-RiPP-like

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Location: 1 - 76,770 nt. (total: 76,770 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00096 - Region 1 - NRPS-like

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Location: 1 - 61,130 nt. (total: 61,130 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 61130:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

9184

AMP-binding (53..363): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GYWHISVLGK
Nearest Stachelhaus code(s):: Pro DIWYISLLGK (29% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_00104 - Region 1 - NRPS,T1PKS

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Location: 1 - 41,343 nt. (total: 41,343 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 41343:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

9449

Search NORINE for peptide: strict or relaxed

PKS_AT (579..889): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 62.5%; Ethylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 73.2
Methoxymalonyl-CoA: 70.7
Isobutyryl-CoA: 70.2
Malonyl-CoA: 38.1
3-Methylbutyryl-CoA: 37.0
Acetyl-CoA: 36.6
Ethylmalonyl-CoA: 35.0
Benzoyl-CoA: 28.1
Propionyl-CoA: 27.6
inactive: 25.4
trans-1,2-CPDA: 21.1
2-Methylbutyryl-CoA: 15.6
fatty_acid: 13.9
CHC-CoA: 13.2

AMP-binding (2886..3298): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLVYYATVQK
Nearest Stachelhaus code(s):: Gly DLVYFATVQK (82% 8� match)
Stachelhaus code match:: 90% (moderate)

scaffold_00106 - Region 1 - T1PKS

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Location: 33,971 - 95,241 nt. (total: 61,271 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

NRPS/PKS monomer predictions

9505

PKS_AT (154..351): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 44.2
Methylmalonyl-CoA: 36.7
Malonyl-CoA: 20.8
Isobutyryl-CoA: 18.2
2-Methylbutyryl-CoA: 16.0
Ethylmalonyl-CoA: 15.0
Benzoyl-CoA: 13.5
CHC-CoA: 12.7
Propionyl-CoA: 12.1
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00121 - Region 1 - NRPS-like

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Location: 1 - 54,450 nt. (total: 54,450 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 54450:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

9913

AMP-binding (39..316): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VAVFIG---K
Nearest Stachelhaus code(s):: Ppa VATFIGGAGK (44% 8� match)
Stachelhaus code match:: 60% (weak)

scaffold_00126 - Region 1 - T1PKS

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Location: 636 - 64,029 nt. (total: 63,394 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 635 - 64029:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk)
Non-colinear ordering:: 10069 + 10068

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10069

PKS_AT (588..897): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 76.6
Methoxymalonyl-CoA: 65.1
Malonyl-CoA: 51.5
Ethylmalonyl-CoA: 37.4
Propionyl-CoA: 35.1
Isobutyryl-CoA: 32.9
trans-1,2-CPDA: 27.1
fatty_acid: 23.9
2-Methylbutyryl-CoA: 19.0
CHC-CoA: 17.1
Acetyl-CoA: 16.6
Benzoyl-CoA: 14.6
3-Methylbutyryl-CoA: 8.7
inactive: -3.2

scaffold_00128 - Region 1 - T1PKS

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Location: 30,914 - 77,948 nt. (total: 47,035 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 30913 - 77948:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10138

PKS_AT (633..955): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Propionyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 75.7
Methoxymalonyl-CoA: 70.9
Malonyl-CoA: 40.7
Ethylmalonyl-CoA: 38.3
Isobutyryl-CoA: 35.3
Propionyl-CoA: 31.2
trans-1,2-CPDA: 27.2
fatty_acid: 21.5
CHC-CoA: 15.1
Benzoyl-CoA: 13.5
2-Methylbutyryl-CoA: 13.4
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00139 - Region 1 - fungal-RiPP-like

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Location: 1 - 55,502 nt. (total: 55,502 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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scaffold_00140 - Region 1 - T1PKS

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Location: 1 - 46,389 nt. (total: 46,389 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Predicted core structure(s)

For candidate cluster 1, location 0 - 46389:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10391

PKS_AT (914..1211): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.0
inactive: 70.9
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 60.7
Propionyl-CoA: 37.9
Isobutyryl-CoA: 19.8
Ethylmalonyl-CoA: 19.7
2-Methylbutyryl-CoA: 18.8
Acetyl-CoA: 16.1
fatty_acid: 14.8
CHC-CoA: 10.2
Benzoyl-CoA: 9.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

scaffold_00143 - Region 1 - NRPS-like,fungal-RiPP-like

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Location: 1 - 65,775 nt. (total: 65,775 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Predicted core structure(s)

For candidate cluster 1, location 0 - 65775:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 20172 - 65775:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10475

AMP-binding (25..337): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ALAFEGMFLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaffold_00144 - Region 1 - T1PKS

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Location: 1 - 64,409 nt. (total: 64,409 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 64409:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10485

PKS_AT (579..902): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Propionyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 89.6
Methoxymalonyl-CoA: 88.9
Malonyl-CoA: 82.2
Ethylmalonyl-CoA: 51.7
Isobutyryl-CoA: 50.6
3-Methylbutyryl-CoA: 31.9
Propionyl-CoA: 30.3
Benzoyl-CoA: 29.4
fatty_acid: 28.0
CHC-CoA: 25.1
inactive: 24.2
trans-1,2-CPDA: 24.1
2-Methylbutyryl-CoA: 16.9
Acetyl-CoA: 15.0

scaffold_00156 - Region 1 - T1PKS

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Location: 1 - 51,445 nt. (total: 51,445 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 51445:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal)
Non-colinear ordering:: 10678 + 10677

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10677

PKS_AT (883..1177): mal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 62.5%; Malonyl-CoA: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.0
Methoxymalonyl-CoA: 53.0
Methylmalonyl-CoA: 45.0
inactive: 41.5
Propionyl-CoA: 24.9
Benzoyl-CoA: 19.5
Isobutyryl-CoA: 18.0
fatty_acid: 16.7
CHC-CoA: 14.9
Acetyl-CoA: 14.8
trans-1,2-CPDA: 13.3
2-Methylbutyryl-CoA: 12.4
Ethylmalonyl-CoA: 11.5
3-Methylbutyryl-CoA: 0.0

10678

PKS_AT (578..890): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 83.0
Methoxymalonyl-CoA: 77.3
Isobutyryl-CoA: 64.3
Malonyl-CoA: 62.5
Ethylmalonyl-CoA: 48.5
Benzoyl-CoA: 30.6
2-Methylbutyryl-CoA: 25.4
inactive: 24.6
trans-1,2-CPDA: 23.8
Propionyl-CoA: 22.2
3-Methylbutyryl-CoA: 18.1
CHC-CoA: 10.2
Acetyl-CoA: 9.2
fatty_acid: 4.1

scaffold_00172 - Region 1 - fungal-RiPP-like

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Location: 1 - 43,382 nt. (total: 43,382 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_00179 - Region 1 - fungal-RiPP-like

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Location: 1 - 41,025 nt. (total: 41,025 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

scaffold_00182 - Region 1 - T1PKS

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Location: 1 - 39,236 nt. (total: 39,236 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 39236:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

10941

PKS_AT (601..941): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%; 2-Methylbutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 74.5
Methoxymalonyl-CoA: 65.9
Propionyl-CoA: 55.3
Malonyl-CoA: 50.2
Ethylmalonyl-CoA: 40.0
Isobutyryl-CoA: 30.6
trans-1,2-CPDA: 28.7
fatty_acid: 28.1
Benzoyl-CoA: 22.3
3-Methylbutyryl-CoA: 17.2
CHC-CoA: 15.1
2-Methylbutyryl-CoA: 15.0
inactive: 13.3
Acetyl-CoA: 0.0

scaffold_00202 - Region 1 - NRPS

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Location: 1 - 31,837 nt. (total: 31,837 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 31837:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

11095

Search NORINE for peptide: strict or relaxed

AMP-binding (74..456): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARGTVSLNK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1100..1488): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DALFTGAMVK
Nearest Stachelhaus code(s):: Leu DALLFGAVVK (65% 8� match)
Stachelhaus code match:: 70% (weak)

scaffold_00244 - Region 1 - fungal-RiPP-like

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Location: 1 - 18,807 nt. (total: 18,807 nt) Show pHMM detection rules used

Region on contig edge.

fungal-RiPP-like: DUF3328

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.